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PCR & RT-PCR

PCR


diamond taq

The wide variety of PCR applications requires occasional adjustments to the PCR process. Optimising PCR for your specific application can make the difference between success and failure. Eurogentec offers you the choice between ready-to-use PCR mixes or separated reagents you can tailor to your needs.

PCR reagent selection guide


 

Ready-to-use PCR Mixes

Polymerases

2X Mixes including:
• Taq® polymerase
• MgCl2
• Buffer
• dNTPs
Components in different vials:
• Taq® polymerase
• MgCl2
• 10X Buffer
Hot start
Reduce unspecific amplification before run starts
NO YES NO YES
Classical activation Short activation
Visualisation
Couloured buffer for direct loading of your PCR on gel
NO YES NO YES NO YES NO NO
Recommended for:

• PCR
• qPCR
• Long template
• GC or AT rich
• Low copy templates


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Specifications

• Yield
• Fidelity
• Specificity


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  GoldStar® Mix Red'y'Gold Mix HotGoldStar Mix Red'y'Star Mix Diamond Taq® SilverStar Red Diamond Taq® Hot Diamond Taq® HGS Diamond Taq®

*Not recommended **Recommended *** Highly recommended
+ Low ++ Medium +++ High

RT-PCR


One-step approach

Two-step approach

  • RT and PCR in a same tube
  • Less contamination risks
  • Less manipulation
  • RT and PCR in separate tubes
  • Ideal for rare sample or multiple targets
  • Core kit including dNTPs
  • Primers
Get RT to add to your Mix    > Get the Reverse Transcriptase Core kit    >


Kits and reagents


Ready-to-use PCR Mixes
DNA Polymerases
dNTP Mix & Set
RT enzyme
Uracil-N-Glycosylase (UNG)
UNG-dUTP blend for carry-over prevention

Plastics


384-well plates
8-tube strips

Dispensing/OEM Services



Assay dispensing service for process automation
OEM Services

Related products



Primers and modified oligonucleotides
Electrophoresis
Nucleic acid purification and extraction kits

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