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Classical immunisation programmes

Classical immunisation programmes

Our classical programmes provide high titre antibodies with excellent affinity. Immunisation lasts from 65 to 90 days depending on the host and the immune response.

The classical programmes are recommended for:

  • Repeating a pre-existing protocol already conducted with a classical programme
  • Immunising a host where the Speedy 28-Day programme is not possible or not guaranteed (chicken, mouse, large animals,…)
  • An antigen of poor immunogenicity that may require prolongation of the programme


Classical immunisation Programmes


Classical immunisation Programmes overview



  Anti-antigen Anti-peptide Double X Anti-PTM*
  You provide the antigena (peptide, protein, complex sample) Eurogentec designsd and synthesises one peptide Eurogentec designs and synthesises two peptides to increase the chances of success Eurogentec designs and synthesises two peptides: one modified with the PTM and one unmodified
Peptide synthesis X 1 peptide
>70% purity
10-16 aa
15-25 mg
2 peptides
>70% purity
10-16 aa
15-25 mg
1 unmodified peptide
>70% purity
10-13 aa
10-20 mg
1 modified peptide (PTM)
>70% purity
10-13 aa
15-20 mg
Coupling to carrier (O)
KLH
BSA
OVA
THY
or MAP carrier
5 mg
Coupled to
KLH
BSA
OVA
THY
or MAP carrier
5 mg of each peptide
Coupled to
KLH
BSA
OVA
THY
or MAP carrier
5 mg of modified peptide
Coupled to
KLH
BSA
OVA
THY
or MAP carrier
Available hostb (Number of host immunised) Rabbit (2)
Rat (2)
Guinea-pig (2)
Mouse (3)
Goat (1)
Chicken (2)
Large animal (1)
Rabbit (2)
Rat (2)
Guinea-pig (2)
Mouse (3)
Goat (1)
Chicken (2)
Large animal (1)
Rabbit (2)
Rat (2)
Guinea-pig (2)
Mouse (3)
Goat (1)
Chicken (2)
Large animal (1)
Rabbit (2)
Rat (6)
Guinea-pig (6)
ELISA Guaranteec X (O) (O) (O)
Affinity purification (O) (O) (O) Double purification of 50 mL serum
QC validation (O) (O) (O) ELISA and SDS-PAGE
Delivery Serum
Purified antibody
(O)
Serum
Purified antibody
(O)
Remaining peptide
Serum
Purified antibody
(O)
Remaining peptides
Purified antibody
Remaining serum
Remaining peptides
 
Request a quote
Request a quote
Request a quote
Request a quote
 
Order form
Order form
Order form
Order form
a. See the section "How to provide your antigen"
b. Other hosts are available on request
c. The guarantee only applies for peptides when approved by Eurogentec
d. Please note that peptide design aims at conferring high antigenicity to the peptides, but doesn’t guaranty that they will lead to antibodies functional in any application. To guarantee an optimal functionality of the generated antibodies in your application, we suggest our DoubleX strategy which involves the design and co-injection of two peptides from the same antigen. This co-immunization more closely mimics the protein than a single peptide strategy and has success rates in the 90 % in Western Blots using this technique.
*Post Translational Modification
X not applicable
(O) optional

How to provide your antigen

Antigen (peptide, protein, complex sample) for classical immunisation programmes can be provided in solution, lyophilised or in gel slice.

The minimal amount of antigen needed depends on the antigen molecular weight and the host as listed in the table below.

Host
(number of host)
Antigen Amount
Antigen MW<18 kDa (µg) Antigen MW>18 kDa (µg)
Per animal and per injection Total amount Per animal per injection Total amount
Mouse (3) 40 480 15 180
Guinea Pig (2) 50 400 30 240
Rat (2) 50 400 30 240
Chicken (2) 200 1600 100 800
Rabbit (2) 200 1600 100 800
Goat (1) 400 1600 200 800
Sheep (1) 400 1600 200 800
Large animal (1) 400 1600 200 800

How to start

1. Fill in the request form
2. Receive a quotation (and recommendations for the peptide sequence in case of anti-peptide production)
3. Complete and send the order form (you will receive the link by email with your quotation)
4. Receive a confirmation email (at this time, your immunisation will start)

For a general discussion about your project or a tailor-made quotation, please contact your sales representative or send your request to proteomics.services@eurogentec.com.

We know that confidentiality is significant for you and we handle your data and project with utmost care and respect.


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