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Custom peptide libraries

Create your own peptide library

Eurogentec proposes custom peptide libraries in a 96-well plate format. Peptide legth is from 5 to 22 amino acids (min 24 peptides). Just send us an Excel® file containing your desired sequences and we will check them for feasibility.

Peptide libraries are available with several modifications, starting with orders of 24 peptides or more.

  • FRET peptide libraries: Peptide libraries are available with Edans-Dabcyl FRET pair labels, the prices indicated are in addition to the cost of the library
  • Phospho peptide libraries: Peptide libraries are available with phosphorylated residues. The prices indicated are in addition to the cost of the library
  • N-terminal Biotin modified peptide libraries: Peptide libraries are available with N-terminal biotin. The prices indicated are in addition to the cost of the library
  • Additional QC by MALDI: An additional quality control by MALDI mass spectrometry can be performed on your library.

Peptide libraries include

  • 200 - 500 µg of each peptide
  • 96-well format
  • Unbound, free crude peptides
  • Purification by selective precipitation (SePop)
  • Lengths between 5-22 amino acids
  • Amino N-terminus
  • CONH2 C-terminus
  • MALDI-TOF QC on 10 % of peptides

Applications

  • 200 - 500 µg of each peptide
  • Epitope mapping
  • Alanine walking
  • Single amino acid mutation screening
  • Protein-protein interaction studies
  • Kinase motif discovery
  • Protease motif discovery

Epitope Mapping

Epitopes recognized by antibodies are commonly 6 amino acids in length. By generating overlapping 15 mer peptides, each shifted by 4 amino acids, one can unequivocally determine which amino acids make up the epitope. Schematically the approach looks as follows based on the following simplified “protein” example:

...KNCSHIQPWETDCLSCLPERQDEYDPKGPKVSDG
1.KNCSHIQPWETDCLS
2........SHIQPWETDCLSCLP
3.............QPWETDCLSCLPERQ
4.....................ETDCLSCLPERQDEY
5...........................CLSCLPERQDEYDPK
6..................................CLPERQDEYDPKGPK
7........................................ERQDEYDPKGPKVSD
8...............................................DEYDPKGPKVSDG
9......................................................DPKGPKVSDG
10...........................................................GPKVSDG

T-Cell Stimulation

Libraries can also be generated to see which particular peptide from an antigenic protein is responsible for a T-cell response. The design process is similar to the epitope mapping example above.

Mutation Studies using Alanine Walking

By screening peptides with systematic replacement of each amino acid with alanine, one can determine to which extent a particular amino acid is required and sufficient for an interaction.

Legal notices: For research use only

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