Catalog Oligonucleotides
Dye-labeled Calibration Oligos
What are calibration oligos?
Dye-labeled calibration oligos are 5’ fluorescent 10-mers dT oligonucleotides used as a reference to calibrate real-time qPCR thermocyclers.
Why use calibration oligos?
Calibration is a preliminary step meant to adjust spectral profiles for each single dye on the qPCR platforms. This allows to compensate for crosstalks between dyes (channels) in the context of multiplexed assays, which is critical for appropriate result analysis.
Specifications
Calibration Oligonucleotides are RP-HPLC purified, MS Quality controled and provided dried at a quantity of 5 nmol.
| Oligo name | Reference | 5' Modification | Abs/Em (nm) | |
|---|---|---|---|---|
| AP5-T10 | UN-CT001-005 | AP5 | 527/549 | • |
| YY-T10 | UN-CT005-005 | Yakima Yellow® | 530/549 | • |
| HEX-T10 | UN-CT010-005 | HEX | 535/556 | • |
| DFO-T10 | UN-CT015-005 | Dragonfly Orange™ | 554/576 | • |
| TET-T10 | UN-CT020-005 | TET | 521/536 | • |
| JOE-T10 | UN-CT025-005 | JOE | 529/555 | • |
| HL647-T10 | UN-CT030-005 | Hilyte™ Fluor 647 | 650/675 | • |
| ROX-T10 | UN-CT035-005 | ROX | 575/602 | • |