Labeling-detection

AnaTag™ AMCA - X Protein Labeling Kit - 1 kit

373.00
Check your price
  • Cat.Number : AS-72055
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Alternative choices

Quantity

AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group. This spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. AMCA fluorescence can be detected at the emission wavelength of 442±10 nm when excited at 353±10 nm. The AMCA-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the AMCA-protein conjugates. The kit is able to perform three conjugation reactions with each reaction labeling up to 5 mg IgG.
Note: AnaTag kits are not intended for peptide labeling.

Specifications

Packaging
Kits components
  • Component A: AMCA-X SE Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 1 mL Component E: 10x Elution buffer for eluting dye-protein conjugate: 30 mL
Properties
Absorbance (nm)
  • 353
Emission (nm)
  • 442
Storage & stability
Storage Conditions
  • Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32