IMG-EN-TAQ-I021-1000-01.jpg
PCR & qPCR

Diamond Taq® DNA Polymerase

€105.00
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  • Cat.Number : TAQ-I021-1000
  • Availability :
    In stock

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Diamond Taq® polymerase is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5’-3’ synthesis of DNA with no detectable 3’-5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning.

Diamond Taq® polymerase is shipped at room temperature and is  particularly recommended for PCR applications that require ultra low levels of bacterial & fungal DNA.


Specifications

Packaging
Kits components
  • TAQ-I021-1000 contains: - One tube (200 µl) of Diamond Taq® DNA Polymerase (concentration > 5 U/µl) - One bottle (6 ml) of reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) - One bottle (6 ml) of MgCl2 solution (25 mM MgCl2). TAQ-I021-5000 contains: - One tube (1 ml) of Diamond Taq® DNA Polymerase (concentration > 5 U/µl) - One bottle (30 ml) of reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) - One bottle (30 ml) of MgCl2 solution (25 mM MgCl2). TAQ-I021-25000- contains: - Five tubes (1 ml each) of Diamond Taq® DNA Polymerase (concentration > 5 U/µl) - Five bottles (30 ml each) of reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) - Five bottles (30 ml each) of MgCl2 solution (25 mM MgCl2).
Chemistry
Molecular Mass/ Weight
  • approx. 95 kDa
Properties
Color
Quantity & Purity
Concentration
  • >= 5 U/µl
Purification Method
  • SDS-PAGE
Storage & stability
Form
  • In solution
Storage Buffer
  • 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer, pH 8.0 (19 °C).
Storage Conditions
  • Storage between -15°C and -25°C is recommended
Activity
Template type
  • DNA
Unit definition
  • One unit is defined as the amount of enzyme that incorporates, 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Usage
  • Research use
Source
Host
Source / Species
  • Thermus aquaticus
Codes
Code Nacres
  • NA.55

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