Image of a kit Takyon Rox SYBR Mmx dTTP blue 1250RXN
PCR & qPCR

Takyon™ ROX SYBR 2X MasterMix dTTP blue

€382.00
Check your price
  • Cat.Number : UF-RSMT-B0701
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Takyon™ qPCR Kits for SYBR® assays containing ROX passive reference are available in different MasterMixes formats from 1.5 ml to 10 x 7.5 ml. Takyon™ qPCR Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & regular conditions

For carry-over prevention, combine any dTTP MasterMix with the new dUTP/UNG additive for 150 rxns: RT-UTPUNG-005, for 750 rxns: RT-UTPUNG-020
To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon™ One-step kit converter for 150 rxns: UF-RTAD-D0101, for 750 rxns: UF-RTAD-D0701

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: One tube (1.5 mL) of 2X MasterMix (blue cap - amber tube) containing Takyon™ DNA polymerase, MgCl2 (2.5mM final concentration), SYBR Green®, dNTPs, ROX Passive reference, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (blue cap - amber vial) containing Takyon™ DNA polymerase, MgCl2 (2.5mM final concentration), SYBR Green®, dNTPs, ROX Passive reference, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use)
Properties
Format
Color
Quantity & Purity
Concentration
  • 2 X
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the Takyon™ Rox SYBR® MasterMix dTTP blue should be stored at a temperature between -15°C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 12 months. For short term storage the Takyon™ Rox SYBR® MasterMix dTTP blue can be stored at 4 °C for 6 months. Product must be stored in the dark.
Activity
qPCR compatible thermocyclers
Application
Template type
  • cDNA/DNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

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frontiers in Immunology . 2021 Nov 12 ; (2020) 11:599625 | DOI : https://doi.org/10.3389/fimmu.2020.599625

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Theoretical and Applied Genetics . 2021 May 02 ; (2021) 134 2495-2515 | DOI : https://doi.org/10.1007/s00122-021-03838-7

  • Peihong Fang
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Lactococcus lactis engineered to deliver hCAP18 cDNA alleviates DNBS-induced colitis in C57BL/6 mice by promoting IL17A and IL10 cytokine expression

Scientific Reports . 2022 Sep 19 ; 12, 15641 (2022) | DOI : https://doi.org/10.1038/s41598-022-19455-3

  • Esther Borras Noguès
  • et al

Rewiring the altered tryptophan metabolism as a novel therapeutic strategy in inflammatory bowel diseases

Gut . 2022 Oct 06 ; (2022) 1–12 | DOI : https://doi.org/10.1136/gutjnl-2022-327337

  • Chloé Michaudel
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Scientific Reports . 2023 Feb 24 ; 3257 (2023) 13 | DOI : https://doi.org/10.1038/s41598-023-29851-y

  • Carolina Manosalva
  • et al

Cytoprotective effects of α-linolenic acid, eicosapentaenoic acid, docosahexaenoic acid, oleic acid and α-tocopherol on 7-ketocholesterol –Induced oxiapoptophagy: Major roles of PI3-K / PDK-1 / Akt signaling pathway and glutathione peroxidase activity in cell rescue

Curr. Res. Toxicol. . 2024 Feb 08 ; Volume 6, 2024 100153 | DOI : https://doi.org/10.1016/j.crtox.2024.100153

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Brain Communications . 2023 Dec 28 ; Volume 6, 1 fcae017 | DOI : https://doi.org/10.1093/braincomms/fcae017

  • Hanna Leister
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Nature Communications . 2024 Feb 05 ; 15 1067 | DOI : https://doi.org/10.1038/s41467-024-45261-8

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  • Katerina Kachler
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  • Zhu Chen
  • Brenda Krishnacoumar
  • Mark Ringer
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