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FRET Peptides

FRET Peptides

FRET (fluorescence resonance energy transfer) based assays have found broad applications, one of which is the detection of protease activity. As a world leader in FRET peptide technology we are proud to offer the same variety of long wavelength quencher and dye pairings used in our ID SensoLyte line of Protease activity assay kits.


Dyes emission colors

Our FRET pairs can be utilised in drug discovery, enabling extensive detection of protease activity to be faster, easier and compatible with HTS. FRET occurs between a peptide tagged to a donor and an acceptor when placed within 10-100 Å of each other resulting in the donor’s excitation fluorescence to be quenched by the acceptor. Enzymatic hydrolysis of the peptide results in recovery of the donor fluorescence following spatial separation of the donor and acceptor upon energy transfer.

FRET peptide


FRET-Peptide specifications

  • Length: 8-10 amino acids between dye and quencher
  • Location: N/C-terminal and internal labelling
  • Quantity: 1-200 mg
  • Purity: 90-95%

FRET-Peptide applications

  • HTS detection of protease activity, protease activators and inhibitors.
  • Protease based drug target screening and discovery protein-peptide interaction.

Peptides with a dye-quencher pair
Dye Ex/Em Quencher
MCA 325/393 nm Dnp
EDANS 335/493 nm DABCYL, DABCYL, PlusTM, QXL® 490
FAM 492/518 nm QXL®520
FITC 494/519 nm QXL®520
HiLyte™ Fluor 488 502/527 nm QXL®520
CyLyte Fluor 3 550/564 nm QXL®570
HiLyte™ Fluor 532 545/565 nm QXL®570
HiLyte™ Fluor 555 550/566 nm QXL®570
TAMRA 541/568 nm QXL®570
Rox 568/591 nm QXL®610
CyLyte Fluor 5 648/663 nm QXL®670
HiLyte™ Fluor 647 650/675 nm QXL®670
CyLyte Fluor 7 750/773 nm IR-QXL®

New Dyes CyLite Fluor

Request a quote

Please complete the Peptide Form to request a quote.
For more information please contact your account Managers or isales@eurogentec.com

 
Request a quote   >
 


Discover our TR-FRET Peptides

A combination of FRET and Time Resolved Fluorescence (TRF) reduce the background noise and increase the sensitivity compared to the classical FRET.

 
Discover the TR-FRET Peptides   >
 

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