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GMP Ultra pure Diamond Taq®

Diamond Taq® family polymerases show very good sensitivity and exceptional reproducibility. As highly thermostable enzymes, they offer an ideal solution for Molecular Diagnostic and demanding Research PCR & qPCR.
The Hot Diamond Taq® represents a completely new “HotStart Concept”. Neither accomplished through chemical modification nor a blocking antibody but a proprietary agent. This polymerase needs a really short activation time and remains compatible with all existing protocols.
These Polymerases are purified from recombinant Escherichia bacteria containing the Thermus aquaticus DNA polymerase gene. This thermophilic eubacterium strain lacks TaqI restriction endonuclease and catalyzes 5’> 3’ synthesis of DNA with no detectable 3’> 5’ exonuclease activity. The enzyme has the 3’ extendase activity allowing TA cloning.

Benefits

GMP compliant

Highly suited for Diagnostic kits, Lab Services (with ASRs) and demanding Research

Ultra-low residual DNA content

<1 fg of genomic E. coli DNA / Taq Unit
Typically < 0.01 fg or 0.002 E. coli genome copies /Taq Unit

Purity

Highly pure (> 98 %) and ultra-low bioburden (≤ 10 CFU/ml). Typically = 0 CFU/ml

Sensitivity
Amplification of DNA templates even at very low concentrations

Exceptional reproducibility

GMP process and stringent QC ensuring lot-to-lot & results reproducibility

Full traceability

Quality Management System fully compliant to ISO 13 485 Medical Device standards and FDA’s Quality System Regulations

Customized fill & finish

Cost-effective tailored solution

> Shipping on dry ice
> Full traceability from production, storage to shipment of the product
> Tracking number sent to customer the day of the shipment
  [shipment at
-20 °C]
   [Sent to customer
the day of the shipment
 
Hot Diamond Taq®: Activation time
Hot Diamond Taq® was evaluated using from 20 seconds to 10 minutes as activation time for its ability to amplify 1 and 5 pg of λDNA. PCR products were analyzed by gel electrophoresis.
Hot Diamond Taq® needs very short activation time [20 sec. are suffi cient]
Diamond Taq® family Spécifications
Parameters Specifications
Appearance* Colorless solution
Identity (SDS-PAGE) MW approx. 95 kDa
Volume activity > 5U/µl
Purity (SDS-PAGE) > 98%
Performance test: PCR-λ DNA* 0.5 kb fragment positive down to 5 pg
Performance test: PCR – 18S DNA* 0.1 kb fragment positive down to 10 pg
Ribonucleases (up to 10U, 1h, 37°C) Not detectable
Endonucleases, Exonucleases, Nicking activity (up to 30U, 16h, 65°C) Not detectable
E.coli residual DNA < 1 fg/Taq Unit
Bioburden* < 10 CFU/ml
Stability* 24 months (at –20°C) from date of manufacture
Animal-derived additives* None
HotStart No detectable amplification without activation
Performance test PCR-Numb DNA (Hot Diamond Taq®) 0.3 kb fragment positive down to 10 pg
* Also for buffer and MgCl2  

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