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Oligonucleotides Aptamers

Oligonucleotides Aptamers

Expert oligonucleotides manufacturer since 1987, Eurogentec collaborates with Novaptech, Dr. Jean-Jacques Toulmé’s french company, one of the world leader in the development of oligonucleotide aptamers.

Eurogentec and Novaptech join their expertise to provide high-value aptamer service which includes the selection and production of custom optimized RNA and DNA aptamers for analytical, diagnostic and therapeutic applications. Each aptamer can be provided in various quantities and delivered either unmodified or modified.

Why oligonucleotide aptamers are into focus?

Oligo Aptamers

Aptamer technology offers an attractive alternative to traditional polyclonal and monoclonal antibodies. Oligonucleotide aptamers are single stranded RNA or DNA sequences folded in a specific 3D conformation. Their tridimensional structure ensures complementarity with their target. The perfect fit between both entities gives to the nucleic acid-based aptamers a high affinity and specificity for a wide range of molecules such as proteins/peptides, nucleic acids, small molecules, toxins, cells and viruses. Aptamers are recognized as promising engineered molecules to target, detect, quantify or capture agents of interest in diagnostic, analytical and therapeutic applications. In addition, due to their small size and high binding affinity, aptamers can be conjugated to therapeutic agents and used as a carrier to deliver the active ingredients directly on site.



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Nucleic acid-based aptamers have multiple advantages



GOOD ALTERNATIVE
TO ANTIBODIES

 

FAST AND CONTROLLED
PRODUCTION

 

ANY TARGET - VARIOUS
CONDITIONS

Similar and even higher affinity and specificity than traditional monoclonal antibodies

Low immunogenicity

Chemical process requiring no animal or cell

Small size (10 times smaller than antibodies)
  Scalable in vitro production

Research and GMP grades

Batch to batch reproducibility
  Effective even against non-immunogenic or toxic targets

High stability at room temperature and resistant to thermal denaturation

Easy chemical modification for improved effciency, even in biological media


Custom RNA and DNA aptamer development

In vitro aptamer selection (SELEX) is an iterative process that generates aptamers with strong binding affinity and high specificity for their targets. Novaptech optimized its aptamer identification process from selection to optimization.

The development process follows a “Go - no Go” approach. At each step a scientific report is generated.



1.
Feasibility Study
2.
Library Screening
3.
Sequence Analysis
4.
Characterization
5.
Optimization
Following customer’s requests, a feasability study is initiated at Novaptech and a quotation is established based on discussions with the customer. Novaptech’s proprietary libraries of up to 1015 random oligonucleotides are screened for interacting sequences.

After several selection rounds, potential interacting candidates are selected.
The selected polyclonal aptamer candidates are analyzed by NGS sequencing and bioinformatic methods. Candidates of interest are identified. A few representative candidates are synthesized at Eurogentec for characterization by biophysical methods. The best monoclonal aptamer sequence is provided to the customer To increase the binding affinity or improve the functionality, the aptamer can be shortened and / or chemically modified

Steps distribution

Novaptech identifies candidates and optimizes aptamers to provide you with a sufficient amount of the selected candidate for initial trials. Logo Novaptech
Eurogentec is committed to synthesizing the final aptamer candidate and all the oligonucleotides required for the development process of your aptamers at Novaptech. Each sequence is produced with the appropriate grade (Research or GMP) according to the final applications ( R&D, Diagnostics or Pre-clinical assays....). Logo Eurogentec

Option

For any upcoming production request Eurogentec will synthesize the oligonucleotide aptamer according to setups defined during the development process. Eurogentec will be able to deliver larger amounts of oligonucleotide aptamers with any chemical modification according to the customer’s needs.



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What are the main applications of nucleic acid-based aptamers?



Antibody-based diagnostics and therapeutics are intensively used in the biomedical field owing to the capability of monoclonal antibodies (mAb) to specifically target and inhibit molecules involved in diseases. However mAb have limitations due to their considerable size, limited stability and costly production.

The main issue for their use as pharmaceutical ingredients remains their potential immunogenicity that could stimulate patient's own immune response. Oligonucleotide aptamers overcome these barriers and are perfectly adapted for diagnostic and therapeutic applications.

Oligo aptamers can be used as:
  • Molecular Probes for Imaging
  • Active therapeutic ingredients
  • Drug carriers
  • Reagents for biosensors
  • Components in biochemical and analytical assays (Affinity chromatography, Cell sorting, ELISA)


   


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