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Positive controls

qPCR & PCR Positive Controls

Eurogentec offers two kinds of control for your quantitative PCR assays.

Positive Controls

Housekeeping controls

Positive controls allow you to detect inhibited amplification reactions due to the carryover of reagents used for the isolation of nucleic acids. Other causes of false-negative results include target nucleic acid degradation, sample processing errors and thermocycler malfunction. Housekeeping controls allow normalisation of gene expression studies. Reliable determination of the gene expression level across samples requires efficient normalisation of the recorded signals. For that purpose Eurogentec has developed a limited set of optimised and validated housekeeping controls. Custom control gene design is available on request.

Benefits

Benefits

  • Specific: Does not interfere with target amplification and avoid amplification of endogenous genes
  • Sensitive: Compatible with low copy templates and available with dark quencher for maximal signal-to-noise ratio
  • Designed for multiplex assays: Detected in the Yakima Yellow®/VIC®/and JOE channel. Validated in duplex assays with the target in the FAM channel


qPCR internal control
  • Validated for human samples
  • Sensitive and specific detection
  • Full expression range coverage
  • Compatible with SYBR® green or probe Assays

IPC

SPC

Catalogue housekeeping control

Custom housekeeping control

The Internal Positive Control (IPC) is spiked into samples before qPCR assay to confirm the absence of target sequence, inhibition or a reaction set-up/cycling error. The Sample Processing Control (SPC) is spiked into samples before extraction to monitor poor extraction yield, PCR inhibition, incorrect pipetting or cycling parameters. Each set has been optimised to a PCR efficiency of 95-100% when combined with a qPCR kit from Eurogentec. You can request your custom design for any other selected reference gene by sending us the gene details
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Positive Control   >
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Processing Control   >
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housekeeping control   >
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design   >


Positive controls


Internal positive control
Sample processing control

Housekeeping controls


Catalogue housekeeping control
Custom housekeeping control

Related products


qPCR Kits and reagents
RT-qPCR Kits and reagents
Primers and modified oligonucleotides
Probes
Electrophoresis
Nucleic acid purification and extraction kits

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