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GMP Diamond Taq®

Diamond Taq® polymerases

Diamond Taq® DNA polymerase is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5’→3’ synthesis of DNA with no detectable 3’→5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning.

Diamond Taq® polymerase is ultra pure polymerase showing very good sensitivity and exceptional reproducibility. It offers an ideal solution for Molecular Diagnostic and is particularly recommended for PCR applications that require ultra low levels of bacterial & fungal DNA and demanding Research fields.

IVD process

  • Shipment on dry ice
  • Hard copy CoA
  • Full traceability from production, storage to shipment
  • Tracking number sent to the customer the day of the shipment
  • Customized Fill & Finish.

Parameters Specifications
Appearance* Colorless solution
Identity (SDS-PAGE) MW approx. 95 kDa
Volume activity ≥ 5 U/µl
Purity (SDS-PAGE) > 98 %
Performance test: PCR - λ DNA* 0.5 kb fragment positive down to 5 pg
Performance test: PCR - 18S DNA* 0.1 kb fragment positive down to 10 pg
Ribonucleases (up to 10 U, 1 h, 37 °C) Not detectable
Endonucleases (up to 30 U, 16 h, 65 °C) Not detectable
Exonucleases (up to 30 U, 16 h, 65 °C) Not detectable
Nicking activity (up to 30 U, 16 h, 65 °C) Not detectable
E. coli residual DNA < 1 fg / Taq Unit
Bioburden* ≤10 CFU/ml
Stability* 24 months (at -20 °C) from date of manufacture
Animal-derived additives* None
* Also for buffer & MgCl2

Package Content

Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
- 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
- MgCl2 solution: 25 mM MgCl2
- Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)

Storage conditions

Storage at -20 °C is recommended

For more information, please contact us.

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