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GMP HGS Diamond Taq®

Diamond Taq® polymerases

HGS Diamond Taq® DNA polymerase is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5’→3’ synthesis of DNA with no detectable 3’→5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning.

HGS Diamond Taq® polymerase HGS Diamond Taq® is a chemically modified HotStart DNA polymerase, which initially lacks all activity below 74 °C. It requires a thermal activation of 10 minutes at 95 °C to reach maximal activity. This avoids non-specific priming at low temperature during the PCR set-up. HGS Diamond Taq® is more heat-stable than commonly used Taq DNA polymerase.

HGS Diamond Taq® DNA polymerase is an ultra pure polymerase preventing non-specific polymerization. HGS polymerase is recommended for multiplex assays.

IVD process

  • Shipment on dry ice
  • Hard copy CoA
  • Full traceability from production, storage to shipment
  • Tracking number sent to the customer the day of the shipment
  • Customized Fill & Finish.


Parameters Specifications
Appearance* Colorless solution
Identity (SDS-PAGE) MW approx. 95 kDa
Volume activity ≥ 5 U/µl
Purity (SDS-PAGE) > 98 %
Performance test: PCR - λ DNA* 0.5 kb fragment positive down to 5 pg
Performance test: PCR - 18S DNA* 0.1 kb fragment positive down to 10 pg
Ribonucleases (up to 10 U, 1 h, 37 °C) Not detectable
Endonucleases (up to 30 U, 16 h, 65 °C) Not detectable
Exonucleases (up to 30 U, 16 h, 65 °C) Not detectable
Nicking activity (up to 30 U, 16 h, 65 °C) Not detectable
E. coli residual DNA < 1 fg / Taq Unit
Bioburden* ≤10 CFU/ml
Stability* 24 months (at -20 °C) from date of manufacture
Animal-derived additives* None
Performance test PCR-Numb DNA 0.3 kb frgment positive down to 10 pg
* Also for buffer & MgCl2

Package Content


HGS Diamond Taq® DNA Polymerase is provided at a concentration > 5 U/µl with Reaction buffer 10x (150 mM Tris-HCl pH 8.5 (at 19°C), 500 mM KCl  and stabilizer) and MgCl2 solution (25 mM MgCl2).

Storage conditions


Storage at -20 °C is recommended.
 

For more information, please contact us.


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