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GMP Hot Diamond Taq®

Diamond Taq® polymerases

Hot Diamond Taq® DNA polymerase is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5’→3’ synthesis of DNA with no detectable 3’→5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning.

Hot Diamond Taq® DNA polymerase exhibits unique Hot Start characteristics and represents a completely new “Hot Start concept”. Hot start characteristics are not accomplished through chemically modification nor a blocking antibody but a proprietary agent prevents non-specific polymerization; thereby preventing primer-dimer formation and increasing the PCR yield of specific products. The enzyme needs very short activation time (100 % activated during the first PCR cycle) but is compatible with all existing protocols (from 20 sec. to 15 min. at 95 °C).

Hot Diamond Taq® DNA polymerase is an ultra pure polymerase preventing non-specific polymerization. HDT polymerase is recommended for long and difficult template amplification.

IVD process

  • Shipment on dry ice
  • Hard copy CoA
  • Full traceability from production, storage to shipment
  • Tracking number sent to the customer the day of the shipment
  • Customized Fill & Finish.

Parameters Specifications
Appearance* Colorless solution
Identity (SDS-PAGE) MW approx. 95 kDa
Volume activity ≥ 5 U/µl
Purity (SDS-PAGE) > 98 %
Performance test: PCR - λ DNA* 0.5 kb fragment positive down to 5 pg
Performance test: PCR - 18S DNA* 0.1 kb fragment positive down to 10 pg
Ribonucleases (up to 10 U, 1 h, 37 °C) Not detectable
Endonucleases (up to 30 U, 16 h, 65 °C) Not detectable
Exonucleases (up to 30 U, 16 h, 65 °C) Not detectable
Nicking activity (up to 30 U, 16 h, 65 °C) Not detectable
E. coli residual DNA < 1 fg / Taq Unit
Bioburden* ≤10 CFU/ml
Stability* 24 months (at -20 °C) from date of manufacture
Animal-derived additives* None
Performance test PCR-Numb DNA 0.3 kb frgment positive down to 10 pg
* Also for buffer & MgCl2

Package Content

Hot Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
- 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
- MgCl2 solution: 25 mM MgCl2
- Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)

Storage conditions

Storage at -20 °C is recommended

For more information, please contact us.

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