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HGS Diamond Taq®

Diamond Taq® polymerases

HGS Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5’→3’ synthesis of DNA with no detectable 3’→5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning.

HGS Diamond Taq® polymerase HGS Diamond Taq® is a chemically modified HotStart DNA polymerase, which initially lacks all activity below 74 °C. It requires a thermal activation of 10 minutes at 95 °C to reach maximal activity. This avoids non-specific priming at low temperature during the PCR set-up. HGS Diamond Taq® is more heat-stable than commonly used Taq DNA polymerase

HGS Diamond Taq® polymerase is particularly recommended for PCR applications that require ultra low levels of bacterial & fungal DNA in Diagnostic and demanding Research fields but also for amplification of templates prone to produce unspecific amplicons.

HGS Diamond Taq® polymerase can be ordered according to your needs :

 

Under IVD process

Under Research process

  • Shipment on dry ice 
  • Hard copy CoA
  • Full traceability from production, storage to shipment
  • Tracking number sent to the customer the day of the shipment
  • Customized Fill & Finish. On request HGS Diamond Taq® can be produced with:
      > An activity from 5 to 50U/μl
      > A glycerol level from 1 to 50 %
  • Shipment at room temperature
  • Full traceability from production to storage of the product

DescriptionSize Reference USD Qty  
HGS Diamond Taq® (sample) - 100 U100 U TAQ-I011-100+ 0.00Add to cart
HGS Diamond Taq - 1000 U1000 U TAQ-I011-1000+ 336.00Add to cart
HGS Diamond Taq - 25000 U25000 U TAQ-I011-25000- 6 753.00Add to cart
HGS Diamond Taq - 5000 U5000 U TAQ-I011-5000+ 1 501.00Add to cart

*now replaces HotGoldStar® brand name

Parameter Specifications
Appearance Colourless solution
Identity (SDS-PAGE) MW approx. 95 kDa
Volume activity ≥ 5 U/µl
Purity (SDS-PAGE) > 98 %
Performance test: PCR on  λ DNA 0.5 kb fragment positive down to 5 pg
Performance test: PCR on genomic DNA - 18S 0.1 kb fragment positive down to 10 pg
Activity 5’-3’ Exonuclease Positive
Ribonucleases (up to 10 U, 1 h, 37 °C) Not detectable
Endoonucleases (up to 10 U, 16 h, 65 °C) Not detectable
Exonucleases (up to 10 U, 16 h, 65 °C) Not detectable
Nicking activity (up to 10 U, 16 h, 65 °C) Not detectable
Hotstart No dectectable amplification without 95°C activation
E. coli residual DNA < 1 fg / Taq Unit
Bioburden ≤10 CFU/ml
Stability 24 months (at -20 °C) from date of manufacture
Animal-derived additives None

Package Content


HGS Diamond Taq® DNA Polymerase is provided at a concentration > 5 U/µl with Reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl  and stabilizer) and MgCl2 solution (25 mM MgCl2).

Storage conditions


Storage at -20 °C is recommended.
 

For more information please dowload the HGS Diamond Taq® brochure


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