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Hot Diamond Taq®

Diamond Taq® polymerases

Hot Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5’→3’ synthesis of DNA with no detectable 3’→5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning.

Hot Diamond Taq® polymerase exhibits unique Hot Start characteristics and represents a completely new “Hot Start concept”. Hot start characteristics are not accomplished through chemically modification nor a blocking antibody but a proprietary agent prevents non-specific polymerization; thereby preventing primer-dimer formation and increasing the PCR yield of specific products. The enzyme needs very short activation time (100 % activated during the first PCR cycle) but is compatible with all existing protocols (from 20 sec. to 15 min. at 95 °C).

Hot Diamond Taq® polymerase is particularly recommended for PCR applications that require ultra low levels of bacterial & fungal DNA in Diagnostic and demanding Research fields but also for amplification of long and difficult templates.

Hot Diamond Taq® polymerase can be ordered according to your needs :

Under IVD process

Under Research process

  • Shipment on dry ice 
  • Hard copy CoA
  • Full traceability from production, storage to shipment.
  • Tracking number sent to the customer the day of the shipment.
  • Customized Fill & Finish. On request Hot Diamond Taq® can be produced with:
      > An activity from 5 to 200 U/μl
      > A glycerol level from 1 to 50 %
  • Shipment on dry ice
  • Full traceability from production to storage of the product.

DescriptionSize Reference USD Qty  
Hot Diamond Taq® (sample)100 U TAQ-I033-100- 0.00Add to cart
Hot Diamond Taq DNA Polymerase1000 U TAQ-I033-1000- 236.00Add to cart
Hot Diamond Taq DNA Polymerase25000 U TAQ-I033-25000- 3 371.00Add to cart
Hot Diamond Taq DNA Polymerase5000 U TAQ-I033-5000 1 011.00Add to cart


Parameter Specifications
Appearance Colorless solution
Identity (SDS-PAGE) MW approx. 95 kDa
Volume activity ≥ 5 U/µl
Purity (SDS-PAGE) > 98 %
Performance test: PCR on λ DNA 0.5 kb fragment positive down to 5 pg
Performance test: PCR on genomic DNA - 18S 0.1 kb fragment positive down to 10 pg
Performance test: PCR on genomic DNA - Numb 0.3 kb fragment positive down to 10 pg
Ribonucleases (up to 10 U, 1 h, 37 °C) Not detectable
Endonucleases (up to 10 U, 16 h, 65 °C) Not detectable
Exonucleases (up to 10 U, 16 h, 65 °C) Not detectable
Nicking activity (up to 10 U, 16 h, 65 °C) Not detectable
E. coli residual DNA < 1 fg / Taq Unit
Bioburden ≤10 CFU/ml
Stability 24 months (at -20 °C) from date of manufacture
Animal-derived additives None


Package Content

Hot Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
- 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
- MgCl2 solution: 25 mM MgCl2
- Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer, pH 8.0 (19 °C).

Storage conditions

Storage at 20 °C is recommended

Literature & Resources 


Diagnostic Taq  Brochure
Research Taq DNA Polymerases Brochure


Regulatory 


IVDT Article on GMP Compliance Oct_Nov 2008
Certification ISO 13485:2003
Certification ISO 9001:2008


Presentations 


Poster at 4th European Congress of Virology 2010
"Novel approaches in manufacturing high quality primers & probes for in-house PCR diagnostics"
Muriel Craynest, Senior Account Manger- Diagnostic services
Presentation at AsiaTides 2010
"Development of a new GMP Taq polymerase for improve PCR performance"
Peter Haima, Ph.D., Director - Diagnostic Services
Marie-Claire Beckers, New Product Development Manager
Presentation at AsiaTides 2009
"Regulatory requirements for diagnostic oligo manufacturing- The value of GMP compliance"
Peter Haima, Ph.D., Director - Diagnostic Services

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