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3' BHQ™ Molecular Beacons

Molecular Beacons

Eurogentec is a licensed supplier of Molecular Beacons, and offers a large number of fluorescent reporters and quenchers. All Molecular Beacons are provided double-HPLC purified. The length of Molecular Beacons with a 3’ BHQ™ should be comprised between 25 to 45 bases.


DescriptionSize Reference USD  
M Beacon 5' TAMRA + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-MB600-004 336.00Configure...
M Beacon 5' TAMRA + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-MB600-020 408.00Configure...
M Beacon 5' TAMRA + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-MB600-100 540.00Configure...
M Beacon 5' FAM + 3' BHQ-1™ 40 nmol scale - 1 probe1 probe PB-MB601-004 312.00Configure...
M Beacon 5' FAM + 3' BHQ-1™ 200 nmol scale - 1 probe1 probe PB-MB601-020 336.00Configure...
M Beacon 5' FAM + 3' BHQ-1™ 1000 nmol scale - 1 probe1 probe PB-MB601-100 492.00Configure...
M Beacon 5' FAM + 3' BHQ-1™ 2.5 µmol scale - 1 probe1 probe PB-MB601-M02 720.00Configure...
M Beacon 5' FAM + 3' BHQ-1™ 5 µmol scale - 1 probe1 probe PB-MB601-M05 1 128.00Configure...
M Beacon 5' FAM + 3' BHQ-1™ 10 µmol scale - 1 probe1 probe PB-MB601-M10 1 849.00Configure...
M Beacon 5' HEX + 3' BHQ-1™ 40 nmol scale - 1 probe1 probe PB-MB610-004 318.00Configure...
M Beacon 5' HEX + 3' BHQ-1™ 200 nmol scale - 1 probe1 probe PB-MB610-020 384.00Configure...
M Beacon 5' HEX + 3' BHQ-1™ 1000 nmol scale - 1 probe1 probe PB-MB610-100 456.00Configure...
M Beacon 5' TET + 3' BHQ-1™ 40 nmol scale - 1 probe1 probe PB-MB612-004 336.00Configure...
M Beacon 5' TET + 3' BHQ-1™ 200 nmol scale - 1 probe1 probe PB-MB612-020 408.00Configure...
M Beacon 5' TET + 3' BHQ-1™ 1000 nmol scale - 1 probe1 probe PB-MB612-100 510.00Configure...
M Beacon 5' JOE + 3' BHQ-1™ 40 nmol scale - 1 probe1 probe PB-MB614-004 336.00Configure...
M Beacon 5' JOE + 3' BHQ-1™ 200 nmol scale - 1 probe1 probe PB-MB614-020 408.00Configure...
M Beacon 5' JOE + 3' BHQ-1™ 1000 nmol scale - 1 probe1 probe PB-MB614-100 540.00Configure...
M Beacon 5' ROX + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-MB616-004 336.00Configure...
M Beacon 5' ROX + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-MB616-020 408.00Configure...
M Beacon 5' ROX + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-MB616-100 540.00Configure...
M Beacon 5'Rhodamine-6G + 3' BHQ-1 40 nmol scale - 1 probe1 probe PB-MB620-004 336.00Configure...
M Beacon 5'Rhodamine-6G+3' BHQ-1 200 nmol scale - 1 probe1 probe PB-MB620-020 408.00Configure...
M Beacon 5'Rhodamine-6G+3' BHQ-1 1000 nmol scale - 1 probe1 probe PB-MB620-100 540.00Configure...
M Beacon 5' Texas Red + 3' BHQ-2 40 nmol scale - 1 probe1 probe PB-MB630-004 336.00Configure...
M Beacon 5' Texas Red + 3' BHQ-2 200 nmol scale - 1 probe1 probe PB-MB630-020 408.00Configure...
M Beacon 5'Texas Red + 3' BHQ-2 1000 nmol scale - 1 probe1 probe PB-MB630-100 540.00Configure...
M Beacon 5'YakimaYellow + 3' BHQ-1 40 nmol scale - 1 probe1 probe PB-MB640-004 384.00Configure...
M Beacon 5'YakimaYellow+3' BHQ-1 200 nmol scale - 1 probe1 probe PB-MB640-020 444.00Configure...
M Beacon 5'YakimaYellow+3' BHQ-1 1000 nmol scale - 1 probe1 probe PB-MB640-100 588.00Configure...
M Beacon 5'YakimaYellow+3' BHQ-1 2.5 µmol scale - 1 probe1 probe PB-MB640-M02 1 008.00Configure...
M Beacon 5'YakimaYellow + 3' BHQ-1 5 µmol scale - 1 probe1 probe PB-MB640-M05 1 681.00Configure...
M Beacon 5'YakimaYellow + 3' BHQ-1 10 µmol scale - 1 probe1 probe PB-MB640-M10 3 001.00Configure...
M Beacon 5' Dragonfly Orange + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-MB645-004 384.00Configure...
M Beacon 5' Dragonfly Orange + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-MB645-020 444.00Configure...
M Beacon 5' Dragonfly Orang + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-MB645-100 564.00Configure...
M Beacon 5DragonflyOrange+3BHQ-2 2.5 µmol scale - 1 probe1 probe PB-MB645-M02 1 008.00Configure...
M Beacon 5'DragonflyOrange+3' BHQ-2 5 µmol scale - 1 probe1 probe PB-MB645-M05 1 681.00Configure...
M Beacon 5DragonflyOrange+3BHQ-2 10 µmol scale - 1 probe1 probe PB-MB645-M10 3 001.00Configure...
M Beacon 5' Cy® 3 + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-MB650-004 On RequestConfigure...
M Beacon 5' Cy® 3 + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-MB650-020 On RequestConfigure...
M Beacon 5' Cy® 3 + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-MB650-100 On RequestConfigure...
M Beacon 5' Cy5® + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-MB655-004 On RequestConfigure...
M Beacon 5' Cy5® + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-MB655-020 On RequestConfigure...
M Beacon 5' Cy5® + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-MB655-100 On RequestConfigure...
M Beacon 5' Cy5® + 3' BHQ-2™ 2.5 µmol scale - 1 probe1 probe PB-MB655-M02 On RequestConfigure...
M Beacon 5' Cy5® + 3' BHQ-2™ 5 µmol scale - 1 probe1 probe PB-MB655-M05 On RequestConfigure...
M Beacon 5' Cy5® + 3' BHQ-2™ 10 µmol scale - 1 probe1 probe PB-MB655-M10 On RequestConfigure...
M Beacon 5' DYOMICS + 3' BHQ™ 40 nmol scale - 1 probe1 probe PB-MB680-004 On RequestConfigure...
M Beacon 5' DYOMICS + 3' BHQ™ 200 nmol scale - 1 probe1 probe PB-MB680-020 On RequestConfigure...
M Beacon 5' DYOMICS + 3' BHQ™ 1000 nmol scale - 1 probe1 probe PB-MB680-100 On RequestConfigure...
M Beacon 5' ATTO + 3' BHQ™ 40 nmol scale - 1 probe1 probe PB-MB690-004 On RequestConfigure...
M Beacon 5' ATTO + 3' BHQ™ 200 nmol scale - 1 probe1 probe PB-MB690-020 On RequestConfigure...
M Beacon 5' ATTO + 3' BHQ™ 1000 nmol scale - 1 probe1 probe PB-MB690-100 On RequestConfigure...
* More info on the Purifications page

More info

Molecular Beacons are probes which contain a stem-loop structure, a fluorophore and a quencher at their 5’ and 3’ ends, respectively.

The ‘stem’ sequence keeps the fluorophore and the quencher together, but only in the absence of a sequence complementary to the ‘loop’ sequence. As long as the fluorophore and the quencher are in close vicinity, any photons emitted by the fluorophore are absorbed by the quencher. This phenomenon is called collisional (or proximal) quenching.

In the presence of a complementary sequence, the probe unfolds and hybridizes to the target, the fluorophore is displaced from the quencher, which can no longer absorb the photons emitted by the fluorophore, and the probe starts to fluoresce. The amount of signal is proportional to the amount of target sequence, and is measured in Real-Time to allow quantification of the amount of target sequence.

Eurogentec is a licensed supplier of Molecular Beacons, and offers a large number of fluorescent reporters and quenchers. All Molecular Beacons are provided double-HPLC purified and controlled by analytical HPLC and MALDI-TOF MS. The maximum length of standard Molecular Beacons with a 3’ BHQ™ is 45 bases. Molecular Beacons are provided lyophilized in individual tubes.

Eurogentec determines upon request the signal-to-noise ratio (STNR) for each Molecular Beacons by hybridizing it to its complementary sequence and comparing fluorescence to background. For FAM labelled Molecular Beacons, the STNR should be > 15. All other dyes require a signal-to-noise ratio > 10. Eurogentec proposes two specialty products: Wavelength-Shifting Molecular Beacons and 2’ O-Methyl RNA Molecular Beacons.

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