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3' Black Hole Quencher™ Double-Dye probes

Doube-Dye LNA Probes

The optimal length of Double-Dye Oligonucleotide probes with a 3’ Dark Quencher (Eclipse® Dark Quencher, or BHQ® or Deep Dark Quencher I or II) is also 25 bases. In case extra long Double-Dye Oligonucleotides probes are required, Deep Dark Quencher II, BHQ-1® and BHQ-2® can be coupled internally.


DescriptionSize Reference USD  
DD Probe 5' TAMRA + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-DD600-004 300.00Configure...
DD Probe 5' TAMRA + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-DD600-020 348.00Configure...
DD Probe 5' TAMRA + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-DD600-100 516.00Configure...
DD Probe 5' FAM + 3' BHQ-1™ 10 nmol scale - 1 probe1 probe PB-DD601-001 174.00Configure...
DD Probe 5' FAM + 3' BHQ-1™ 40 nmol scale - 1 probe1 probe PB-DD601-004 210.00Configure...
DD Probe 5' FAM + 3' BHQ-1™ 200 nmol scale - 1 probe1 probe PB-DD601-020 288.00Configure...
DD Probe 5' FAM + 3' BHQ-1™ 1000 nmol scale - 1 probe1 probe PB-DD601-100 462.00Configure...
DD Probe 5' FAM + 3' BHQ-1™ 2.5 µmol scale - 1 probe1 probe PB-DD601-M02 948.00Configure...
DD Probe 5' FAM + 3' BHQ-1™ 5 µmol scale - 1 probe1 probe PB-DD601-M05 1 333.00Configure...
DD Probe 5' FAM + 3' BHQ-1™ 10 µmol scale - 1 probe1 probe PB-DD601-M10 2 221.00Configure...
DD Probe 5'FAM + 3' BHQ-1™ 20 µmol scale - 1 probe1 probe PB-DD601-M20 3 241.00Configure...
DD Probe 5' HEX + 3' BHQ-1™ 40 nmol scale - 1 probe1 probe PB-DD610-004 211.00Configure...
DD Probe 5' HEX + 3' BHQ-1™ 200 nmol scale - 1 probe1 probe PB-DD610-020 256.00Configure...
DD Probe 5' HEX + 3' BHQ-1™ 1000 nmol scale - 1 probe1 probe PB-DD610-100 387.00Configure...
DD Probe 5' TET + 3' BHQ-1™ 40 nmol scale - 1 probe1 probe PB-DD612-004 216.00Configure...
DD Probe 5' TET + 3' BHQ-1™ 200 nmol scale - 1 probe1 probe PB-DD612-020 264.00Configure...
DD Probe 5' TET + 3' BHQ-1™ 1000 nmol scale - 1 probe1 probe PB-DD612-100 402.00Configure...
DD Probe 5' JOE + 3' BHQ-1™ 40 nmol scale - 1 probe1 probe PB-DD614-004 288.00Configure...
DD Probe 5' JOE + 3' BHQ-1™ 200 nmol scale - 1 probe1 probe PB-DD614-020 360.00Configure...
DD Probe 5' JOE + 3' BHQ-1™ 1000 nmol scale - 1 probe1 probe PB-DD614-100 480.00Configure...
DD Probe 5' ROX + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-DD616-004 288.00Configure...
DD Probe 5' ROX + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-DD616-020 360.00Configure...
DD Probe 5' ROX + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-DD616-100 480.00Configure...
DD Probe 5' Rhodamine-6G + 3' BHQ-1™ 40 nmol scale - 1 probe1 probe PB-DD620-004 282.00Configure...
DD Probe 5' Rhodamine-6G + 3' BHQ-1™ 200 nmol scale - 1 probe1 probe PB-DD620-020 324.00Configure...
DD Probe 5' Rhodamine-6G + 3' BHQ-1™ 1000 nmol scale - 1 probe1 probe PB-DD620-100 498.00Configure...
DD Probe 5' Texas Red® + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-DD630-004 282.00Configure...
DD Probe 5' Texas Red® + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-DD630-020 324.00Configure...
DD Probe 5' Texas Red® + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-DD630-100 478.00Configure...
DD Probe 5' Yakima Yellow® + 3' BHQ-1™ 40 nmol scale - 1 probe1 probe PB-DD640-004 312.00Configure...
DD Probe 5' Yakima Yellow® + 3' BHQ-1™ 200 nmol scale - 1 probe1 probe PB-DD640-020 376.00Configure...
DD Probe 5' Yakima Yellow® + 3' BHQ-1™ 1000 nmol scale - 1 probe1 probe PB-DD640-100 524.00Configure...
DDProbe 5'YakimaYellow + 3' BHQ-1™ 2.5 µmol scale - 1 probe1 probe PB-DD640-M02 854.00Configure...
DDProbe 5'YakimaYellow + 3' BHQ-1™ 5 µmol scale - 1 probe1 probe PB-DD640-M05 1 441.00Configure...
DDProbe 5'YakimaYellow + 3' BHQ-1™ 10 µmol scale - 1 probe1 probe PB-DD640-M10 2 425.00Configure...
DD Probe 5' Dragonfly Orange' + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-DD645-004 348.00Configure...
DD Probe 5' Dragonfly Orange' + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-DD645-020 414.00Configure...
DD Probe 5' Dragonfly Orange' + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-DD645-100 540.00Configure...
DDProbe 5' DragonflyOrange+3' BHQ-2™ 2.5 µmol scale - 1 probe1 probe PB-DD645-M02 900.00Configure...
DDProbe 5' DragonflyOrange+3' BHQ-2™ 5 µmol scale - 1 probe1 probe PB-DD645-M05 1 441.00Configure...
DDProbe 5' DragonflyOrange+3' BHQ-2™ 10 µmol scale - 1 probe1 probe PB-DD645-M10 2 437.00Configure...
DD Probe 5' Cy® 3 + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-DD650-004 276.00Configure...
DD Probe 5' Cy® 3 + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-DD650-020 324.00Configure...
DD Probe 5' Cy® 3 + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-DD650-100 522.00Configure...
DD Probe 5' Cy5® + 3' BHQ-2™ 40 nmol scale - 1 probe1 probe PB-DD655-004 270.00Configure...
DD Probe 5' Cy5® + 3' BHQ-3™ 40 nmol scale - 1 probe1 probe PB-DD655-004B 564.00Configure...
DD Probe 5' Cy5® + 3' BHQ-2™ 200 nmol scale - 1 probe1 probe PB-DD655-020 318.00Configure...
DD Probe 5' Cy5® + 3' BHQ-3™ 200 nmol scale - 1 probe1 probe PB-DD655-020B 768.00Configure...
DD Probe 5' Cy5® + 3' BHQ-2™ 1000 nmol scale - 1 probe1 probe PB-DD655-100 510.00Configure...
DD Probe 5' Cy5® + 3' BHQ-3™ 1000 nmol scale - 1 probe1 probe PB-DD655-100B 960.00Configure...
DD Probe 5' Cy5® + 3' BHQ-2™ 2.5 µmol scale - 1 probe1 probe PB-DD655-M02 876.00Configure...
DD Probe 5' Cy5® + 3' BHQ-2™ 5 µmol scale - 1 probe1 probe PB-DD655-M05 1 321.00Configure...
DD Probe 5' Cy5® + 3' BHQ-2™ 10 µmol scale - 1 probe1 probe PB-DD655-M10 2 221.00Configure...
DD Probe 5' DYOMICS + 3' BHQ™ 40 nmol scale - 1 probe1 probe PB-DD680-004 On RequestConfigure...
DD Probe 5' DYOMICS + 3' BHQ™ 200 nmol scale - 1 probe1 probe PB-DD680-020 On RequestConfigure...
DD Probe 5' DYOMICS + 3' BHQ™ 1000 nmol scale - 1 probe1 probe PB-DD680-100 On RequestConfigure...
DD Probe 5' ATTO + 3' BHQ™ 40 nmol scale - 1 probe1 probe PB-DD690-004 324.00Configure...
DD Probe 5' ATTO + 3' BHQ™ 200 nmol scale - 1 probe1 probe PB-DD690-020 402.00Configure...
DD Probe 5' ATTO + 3' BHQ™ 1000 nmol scale - 1 probe1 probe PB-DD690-100 541.00Configure...
More info on the Purifications page

More info

Double-Dye Oligonucleotides have a fluorescent reporter dye and a quencher at their 5’ and 3’ ends, respectively.

Double-Dye Oligonucleotides are designed to anneal between the upstream and downstream PCR primers. The Tm of the Double-Dye Oligonucleotide probe must be higher than the upstream primer, to avoid a situation where the later primer is already being extended while the Double-Dye Oligonucleotide probe is not yet annealed.

During the amplification process, the 5’-> 3’ exonuclease activity of the Taq DNA polymerase cleaves the fluorophore from the probe. Since the fluorophore is no longer subjected to FRET quenching, it starts to fluoresce. This fluorescence can be measured, and the level is directly proportional to the amount of target DNA accumulating during the PCR reaction.

Eurogentec is a supplier of Double-Dye Oligonucleotide probes and offers the largest number of fluorescent reporters and quenchers. Double-Dye Oligonucleotide probes with TAMRA or various non-fluorescent quenchers (e.g. the Black Hole Quencher® family) and a variety of fluorescent dyes can be conveniently ordered using our web ordering interface. Furthermore, as many applications, like genotyping or gene expression profiling, are requiring small amounts of several different probes, Eurogentec has developed a high-throughput platform to produce high quality small scale Real-Time qPCR Double-Dye probes.

All Double-Dye Oligonucleotides are HPLC purified. They are controlled by analytical HPLC and MALDI-TOF Mass Spectrometry. Double-Dye Oligonucleotides are provided lyophilized in individual tubes. The optimal recommended length of Double-Dye Oligonucleotide probes is 25 bases. The maximum length of Double-Dye Oligonucleotides is 35 bases. In case of extra long Double-Dye Oligonucleotides probes are required, quenchers like Black Hole Quencher®, Deep Dark Quencher I and TAMRA can be coupled internally.

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