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3' Eclipse® Dark Quencher Double-Dye probes

Doube-Dye LNA Probes

The optimal length of Double-Dye Oligonucleotide probes with a 3’ Eclipse® Dark Quencher is 25 bases.

DescriptionSize Reference USD  
DD Probe 5' FAM + 3' EDQ 40 nmol scale - 1 probe1 probe PB-DD301-004 198.00Configure...
DD Probe 5' FAM + 3' EDQ 200 nmol scale - 1 probe1 probe PB-DD301-020 258.00Configure...
DD Probe 5' FAM + 3' EDQ 1000 nmol scale - 1 probe1 probe PB-DD301-100 402.00Configure...
DD Probe 5' HEX + 3' EDQ 40 nmol scale - 1 probe1 probe PB-DD310-004 222.00Configure...
DD Probe 5' HEX + 3' EDQ 200 nmol scale - 1 probe1 probe PB-DD310-020 276.00Configure...
DD Probe 5' HEX + 3' EDQ 1000 nmol scale - 1 probe1 probe PB-DD310-100 432.00Configure...
DD Probe 5' TET + 3' EDQ 40 nmol scale - 1 probe1 probe PB-DD312-004 216.00Configure...
DD Probe 5' TET + 3' EDQ 200 nmol scale - 1 probe1 probe PB-DD312-020 258.00Configure...
DD Probe 5' TET + 3' EDQ 1000 nmol scale - 1 probe1 probe PB-DD312-100 450.00Configure...
DD Probe 5' JOE + 3' EDQ 40 nmol scale - 1 probe1 probe PB-DD314-004 300.00Configure...
DD Probe 5' JOE + 3' EDQ 200 nmol scale - 1 probe1 probe PB-DD314-020 354.00Configure...
DD Probe 5' JOE + 3' EDQ 1000 nmol scale - 1 probe1 probe PB-DD314-100 516.00Configure...
DD Probe 5' Rhodamine-6G + 3' EDQ 40 nmol scale - 1 probe1 probe PB-DD320-004 300.00Configure...
DD Probe 5' Rhodamine-6G + 3' EDQ 200 nmol scale - 1 probe1 probe PB-DD320-020 354.00Configure...
DD Probe 5' Rhodamine-6G + 3' EDQ 1000 nmol scale - 1 probe1 probe PB-DD320-100 516.00Configure...
DD Probe 5' Yakima Yellow® + 3' EDQ 40 nmol scale - 1 probe1 probe PB-DD340-004 300.00Configure...
DD Probe 5' Yakima Yellow® + 3' EDQ 200 nmol scale - 1 probe1 probe PB-DD340-020 348.00Configure...
DD Probe 5' Yakima Yellow® + 3' EDQ 1000 nmol scale - 1 probe1 probe PB-DD340-100 516.00Configure...
DD Probe 5' AP5 + 3' EDQ 1000 nmol scale - 1 probe1 probe PB-DD350-100 624.00Configure...
DD Probe 5' DYOMICS + 3' EDQ 40 nmol scale - 1 probe1 probe PB-DD380-004 On RequestConfigure...
DD Probe 5' DYOMICS + 3' EDQ 200 nmol scale - 1 probe1 probe PB-DD380-020 On RequestConfigure...
DD Probe 5' DYOMICS + 3' EDQ 1000 nmol scale - 1 probe1 probe PB-DD380-100 On RequestConfigure...
DD Probe 5' ATTO + 3' EDQ 40 nmol scale - 1 probe1 probe PB-DD390-004 330.00Configure...
DD Probe 5' ATTO + 3' EDQ 200 nmol scale - 1 probe1 probe PB-DD390-020 417.00Configure...
DD Probe 5' ATTO + 3' EDQ 1000 nmol scale - 1 probe1 probe PB-DD390-100 555.00Configure...
More info on the Purifications page

More info

Double-Dye Oligonucleotides have a fluorescent reporter dye and a quencher at their 5’ and 3’ ends, respectively.

Double-Dye Oligonucleotides are designed to anneal between the upstream and downstream PCR primers. The Tm of the Double-Dye Oligonucleotide probe must be higher than the upstream primer, to avoid a situation where the later primer is already being extended while the Double-Dye Oligonucleotide probe is not yet annealed.

During the amplification process, the 5’ > 3’ exonuclease activity of the Taq DNA polymerase cleaves the fluorophore from the probe. Since the fluorophore is no longer subjected to FRET quenching, it starts to fluoresce. This fluorescence can be measured, and the level is directly proportional to the amount of target DNA accumulating during the PCR reaction.

Eurogentec is a supplier of Double-Dye Oligonucleotide probes and offers the largest number of fluorescent reporters and quenchers. Double-Dye Oligonucleotide probes with TAMRA or various non-fluorescent quenchers (e.g. the Black Hole Quencher™ family) and a variety of fluorescent dyes can be conveniently ordered using our web ordering interface. Furthermore, as many applications, like genotyping or gene expression profiling, are requiring small amounts of several different probes, Eurogentec has developed a high-throughput platform to produce high quality small scale Real-Time qPCR Double-Dye probes.

All Double-Dye Oligonucleotides are HPLC purified. They are controlled by analytical HPLC and MALDI-TOF Mass Spectrometry. Double-Dye Oligonucleotides are provided lyophilized in individual tubes. The optimal recommended length of Double-Dye Oligonucleotide probes is 25 bases. The maximum length of Double-Dye Oligonucleotides is 35 bases. In case of extra long Double-Dye Oligonucleotides probes are required, quenchers like Black Hole Quencher™, Deep Dark Quencher I and TAMRA can be coupled internally.

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