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Acetylcholinesterase Activity Assay Kit

iD Sensolyte® Assay Kits


Acetylcholinesterase (AChE) hydrolyses acetylcholine into choline and acetic acid. Alzheimers and myastenia gravis diseases are characterized by reduced acetylcholine. Thus, drugs that inhibit AChE were developed to reduce disease symptoms.4

The SensoLyte® 520 Acetylcholinesterase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of AChE inhibitors. SensoLyte® 520 Acetylcholinesterase Assay Kit can detect as low as 8 pg/ml of active AChE.

• Optimized Performance: This kit is optimized to detect human acetylcholinesterase activity
• Enhanced Value: It provides ample reagents to perform 200 assays in a 96-well format
• High Speed: The entire process can be completed in 1 hour
• Assured Reliability: Detailed protocol and references are provided

DescriptionSize Reference USD Qty  
SensoLyte® 520 Acetylcholinesterase Assay Kit *Fluorimetric* - 1 kit1 kit AS-72242 286.00Add to cart


Other Materials Required (but not provided)

  • 96-well microplate: Black, flat bottom 96-well plate with non-binding surface
  • Fluorescence microplate reader: Capable of detecting emission at 520 nm with excitation at 490 nm
  • Plate cover: To prevent liquid evaporation during incubation step

Storage and Handling

  • Store kit Components A, C, D, and E at -20°C
  • Store Component B at -80°C
  • Protect Component A from light and from moisture

Acetylcholinesterase (AChE) is a fast acting serine hydrolase that belongs to the carboxylesterase enzyme family.1-4 It hydrolyses acetylcholine into choline and acetic acid and is found in the synaptic cleft. It can be detected in many mammals and non-mammalian species.1-4 Alzheimers and myastenia gravis diseases are characterized by reduced acetylcholine. Thus, drugs that inhibit AChE were developed to reduce disease symptoms.4 AChE can be inhibited by many natural substances such as alkaloids and synthetic chemicals such as organophosphorous compounds that are frequently used as pesticides.2-4 Recently, it was demonstrated that natural compounds have variable inhibitory effect on AChE depending on the species.5

The SensoLyte® 520 Acetylcholinesterase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of AChE inhibitors. Active AChE generates fluorescence that can be monitored at excitation/emission=490/520 nm. The long wavelength fluorescence of the substrate is less interfered by the autofluorescence of components in biological samples and test compounds. SensoLyte® 520 Acetylcholinesterase Assay Kit can detect as low as 8 pg/ml of active AChE.


Measurement of Acetylcholinesterase activity with the AChE SensoLyte Assay kit from AnaSpec
Figure 1. Measurement of AChE activity in human cerebrospinal fluid (CSF was diluted at 1:10 ratio with the Assay Buffer) in the presence or absence of AChE inhibitor (5µM neostigmine bromide) using SensoLyte® 520 Acetyl Cholinesterase Activity Assay Kit (Cat# AS-72242).



References

  • 1. Tong F., et.al., Pesticide Biochem and Phys 106 (2013): 79-84
  • 2. Kozurkova M., et.al., Pharmaceuticals 4 (2011): 382-418
  • 3. Lee S. R., et.al., Mar Drugs 12 (2014): 3560-3573
  • 4. Pohanka M, Int J Mol Sci 15 (2014): 9809-9825
  • 5. Ramli R. A., et.al., Nat Prod Commun 8 (2013): 695-698

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