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Aggrecanase-1 Assay Kits

iD Sensolyte® Assay Kits

Aggrecanases belong to ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases. Aggrecanases cleave aggrecan, the major structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a major aggrecanase in human osteoarthritic cartilage. 

The SensoLyte® 520 Aggrecanase-1 Assay Kit uses 5-FAM as the fluorophore and TAMRA as the quencher in the FRET peptide substrate. This substrate is used for continuous measurement of the enzyme activity. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by TAMRA. Upon cleavage of the FRET peptide by ADAMTS-4, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Increase in FAM fluorescence is correlated to aggrecanase-1 activity. 5-FAM/TAMRA based FRET substrate presents a limited interference by auto-fluorescence of test compounds due to its longer emission wavelength. The kit can be used to detect the activity of aggrecanase-1 or screen for aggrecanase inhibitors. The assays are performed in a convenient 96-well format.

DescriptionSize Reference USD Qty  
SensoLyte® 520 Aggrecanase-1 Assay Kit *Fluorimetric* - 1 kit1 kit AS-72114 457.00Add to cart

Description



Assay kit λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
SensoLyte® 520 Aggrecanase-1 Assay Kit   490/520  50 μL 0.1 ng  • Aggrecanase-1 substrate, 5-FAM/5-TAMRA FRET peptide, (the cleavage of the substrate by other members of aggrecanase family, such as ADAMTS-1 and ADAMTS-5, is negligible)
• 5-FAM, fluorescence reference standard
• HeLa nuclear extract
• Assay buffer
• Control Inhibitor
• Stop Solution
1 hour 100



Performance
: This kit is optimized to detect Aggrecanase-1 activity

Value: It provides enough reagents to perform 100 assays in a 96-well format 

High Speed: The entire process can be completed in one hour 

Reliability: Detailed protocol and references are provided  

Storage: -20°C




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All oligonucleotides
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You can still place orders by:
orders.anaspec@egt-biotech.com
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