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Alpha-Secretase Assay Kits

iD Sensolyte® Assay Kits

Secretases are key enzymes involved in the production of beta-amyloid peptides found in extracellular amyloid plaques of Alzheimer's disease (AD). They are considered excellent targets for anti-amyloid treatment of Alzheimer's disease. In healthy brains, Amyloid Precursor Protein (APP) is predominantly processed by TACE (tumor necrosis factor-alpha converting enzyme, ADAM17), producing a 83 amino acid C-terminal fragment. TACE is a member of the ADAM family of proteases that contain both disintegrin and metalloprotease (catalytic) domains. This enzyme is involved in sperm-egg binding and fusion, muscle cell fusion, neurogenesis, modulation of Notch receptor and ligand processing, and processing of the pro-inflammatory cytokine, TNFα. TACE is currently being explored as a target for anti-inflammatory drugs.

ADAM10 has been shown to be an effective α-secretase in vitro and in vivo. This enzyme acts as a sheddase to cleave cell surface proteins involved in neuropathology, inflammatory response and tumor progression.

The SensoLyte® 520 TACE Activity Assay Kit uses a 5-FAM (fluorophore) and QXL™ 520 (quencher) labeled FRET peptide substrate for continuous measurement of enzyme activity. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™ 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The kit can be used to detect the activity of TACE or screen for TACE inhibitors. The assay can detect as low as 3.1 ng/mL active TACE.

The SensoLyte® 520 ADAM10 Activity Assay Kit is an optimized assay that can be used to detect the ADAM 10 activity. The unique FRET substrate contained in the kit was designed to reduce the cross reactivity with TACE/ADAM17. When active ADAM10 cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence monitored at excitation/emission = 490/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.25 ng/mL active ADAM10.

DescriptionSize Reference USD Qty  
SensoLyte® 520 ADAM10 Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72226 501.00Add to cart
SensoLyte® 520 TACE (a-Secretase) Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72085 457.00Add to cart

  • Convenient format: These kits provide ample reagents to perform 100 assays in a 96-well plate format
  • High Speed: The entire process can be completed in one hour
  • Assured Reliability: Detailed protocol and references are provided
  • High sensitivity: The assays can detect as low as 3.1 ng/ml ADAM17 or 0.25 ng/mL active ADAM10

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