The AnaTag™ Protein Labeling Kits provide a convenient way to label proteins using the succinimidyl ester (SE) reactive form of the HiLyte™ Fluor dyes. The succinimidyl ester shows good reactivity and selectivity with aliphatic amines of the protein and forms a carboxamide bond, which is identical to, and is as stable as the natural peptide bond.
HiLyte™ Fluor- protein conjugates may be used for immunofluorescent staining, fluorescent in situ hybridization, flow cytometry and other biological applications. Each kit has all the essential components for performing the conjugation reaction and for purifying the HiLyte™ Fluor- protein conjugates.
Figure 1 : The succinimidyl ester (SE) group of the fluorophore reacts with the amino group of lysines on the protein to form a stable carboxamide bond.
Figure 2 : Labeling of an amino group (for instance, side chain of lysine) on a biopolymer with a succinimidyl ester of a dye in 4 easy steps using the AnaTag™ Protein Labeling Kits.