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Angiotensin-Converting Enzyme 2 Assay Kits

iD Sensolyte® Assay Kits

Angiotensin-converting enzyme 2 (ACE2), a homolog of angiotensin-converting enzyme (ACE), is a zinc metallopeptidase with specificity, tissue distribution, and function distinct from those of ACE. ACE2 may play a unique role in the renin-angiotensin system and mediate cardiovascular and renal functions. 

The SensoLyte® 390 ACE2 Activity Assay Kit provides a convenient assay for high throughput screening of ACE2 enzyme inhibitors and inducers using a Mc-Ala/Dnp fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide, the fluorescence of Mc-Ala is quenched by Dnp. Upon cleavage into two separate fragments by ACE2, the fluorescence of Mc-Ala is recovered, and can be monitored at excitation/emission = 330/390 nm. This assay can detect the activity of subnanogram levels of ACE2. Assays are performed in a convenient 96-well microplate format.

DescriptionSize Reference USD Qty  
SensoLyte® 390 ACE2 Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72086 457.00Add to cart

Description



λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Angiotensin Converting Enzyme 2 Assay Kit
SensoLyte® 390 ACE2 Activity
Fluorimetric
330/390 50 µl 0.05 ng/well • ACE2 substrate: Mca/Dnp FRET peptide
• Mca fluorescence reference standard
• Assay Buffer
• Inhibitor of ACE2
• Stop Solution
60 min. 100

Performance: This kit is optimized to detect ACE2 activity 

Enhanced Value: It provides enough reagents to perform 100 assays in a 96-well format 

High Speed: The entire process can be completed in one hour 

Reliability: Detailed protocol and references are provided

Storage: -20°C
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