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Beta-Galactosidase Assay Kits

iD Sensolyte® Assay Kits

β-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporter enzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells. 

The SensoLyte® MUG β-Galactosidase Assay Kit provides a highly sensitive fluorogenic substrate for detection of β-galactosidase activity in tranfected cells. In the presence of β-galactosidase, the colorless 4-methylumbelliferyl β-D-galactopyranoside (MUG) substrate is hydrolyzed into a product that has bright blue fluorescence. Fluorescence can then be detected at Ex/Em = 360/460 nm. 

The SensoLyte® FDG β-Galactosidase Assay Kit provides a sensitive fluorogenic substrate for detecting β-galactosidase activity in tranfected cells. In the presence of β-galactosidase, the colorless fluorescein di-β-D galactopyranoside (FDG) substrate is hydrolyzed into a fluorescein that can be detected at Ex/Em = 490/520 nm. 

The SensoLyte® ONPG β-Galactosidase Assay Kit utilizes the widely used chromogenic substrate, o-nitrophenyl β-D-galactopyranoside (ONPG) to determine the activity of β-galactosidase in transfected cells or tissues. In the presence of β-galactosidase, the colorless compound is hydrolyzed into o-nitrophenol, producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to β-galactosidase activity.

DescriptionSize Reference USD Qty  
SensoLyte® FDG b-Galactosidase Assay Kit *Fluorimetric* - 1 kit1 kit AS-72133 286.00Add to cart
SensoLyte® MUG b-Galactosidase Assay Kit *Fluorimetric* - 1 kit1 kit AS-72132 286.00Add to cart
SensoLyte® ONPG b-Galactosidase Assay Kit *Colorimetric* - 1 kit1 kit AS-72134 210.00Add to cart

Description



λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
ß-Galactosidase Assay Kit
SensoLyte® FDG ß-Galactosidase Fluorimetric 490/520 10 µl 10 pg • ß-galactosidase substrate: FDG (fluorescein di-ß-D-galactopyranoside)
• Fluorescein, reference standard
• ß-galactosidase enzyme
• Assay buffer
• DTT
• Triton X-100
• Stop solution
30 min 500
SensoLyte® MUG ß-Galactosidase Fluorimetric 360/460 10 µl 15 fg

• ß-galactosidase substrate: MUG (4-methylumbelliferyl ß-D-galactopyranoside)
• 4-MU, reference standard
• ß-galactosidase enzyme
• Assay buffer
• DTT
• Triton X-100
• Stop solution

30 min 500
SensoLyte® ONPG ß-Galactosidase Colorimetric Abs
420
10 µl 0.1 ng •  ß-galactosidase substrate: ONPG (o-nitrophenyl ß-D-galactopyranoside)
• ß-galactosidase enzyme
• Lysis buffer
• Stop solution
1 hour 100

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