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Calpain Assay Kits

iD Sensolyte® Assay Kits

The calpains are a family of intracellular Ca2+ dependent cysteine proteases. The best-characterized calpains are isoforms 1 (μ) and 2 (m). They respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). Calpains are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer’s disease, and cancer. Calpains represent potential therapeutic targets for drug discovery.

The SensoLyte® 520 Calpain Assay Kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate. Calpain protease cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.

The SensoLyte® AMC Calpain Assay Kit contains a fluorogenic peptide substrate, suc-LLVY-AMC. Upon calpain protease cleavage, suc-LLVY-AMC generates the AMC fluorophore emitting bright blue fluorescence and can be monitored at excitation/emission=354/442 nm.

In both assays, increase in AMC fluorescence is proportional to calpain activity. The assays can detect both calpain 1 (μ) and 2 (m) activities and are ideal for kinetic study of these enzymes. The assays are performed in a convenient 96-well microplate format

DescriptionSize Reference USD Qty  
SensoLyte® 520 Calpain Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72149 501.00Add to cart
SensoLyte® AMC Calpain Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72150 497.00Add to cart


Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Calpain 1 and 2 (μ and m)
SensoLyte® 520
Calpain Assay
490/520 50 μl 3 ng/well • Calpain 1 and 2 substrate: 5-FAM/QXL™520-based FRET peptide
• Assay buffer
• Human Calpain (positive control)
• Calpain Inhibitor
• Fluorescence reference standard for calibration (5-FAM)
1 hour 100
SensoLyte® AMC Calpain Assay
354/442 50 μl 15 ng/well • Calpain 1 and 2 substrate: suc-LLVY-AMC
• Assay Buffer
• Human Calpain (positive control)
• Calpain Inhibitor
• Fluorescence reference standard for calibration (AMC)
1 hour 100

: These kits are optimized to detect calpain-1 and -2 activity 

Enhanced Value: Enough reagents are provided to perform 100 assays in a 96-well format

High Speed: The entire process can be completed in one hour 

Reliability: Detailed protocol and references are provided 

Storage: -80°C


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