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Caspase Assay Kits

iD Sensolyte® Assay Kits

Apoptosis, a programmed, cell-autonomous death process, plays important roles in a variety of physiological and pathological events. These range from normal fetal development to diseases, such as cancer, organ failure and neurodegenerative diseases. During apoptosis, caspases execute the disassembly of cellular components by proteolytic cleavage of a variety of substrates, such as poly-(ADP ribose) polymerase, DNA-dependent protein kinase (DNA-PK), topoisomerases, and protein kinase C delta. At least ten caspases have been discovered. Some of these caspases identify and cleave a specific peptide substrate, while others recognize the same peptide substrate. They are also identified as drug-screening targets. 

Caspase-8, also known as FLICE, MACH and Mch5, is a key enzyme in the apoptosis pathway. It is an upstream caspase that accepts signals from death receptors and initiates the apoptosis pathways. Caspase-8 cleaves inactive pro-forms of effector caspase-3, -6 and –7, thereby activating them. These caspases are then capable of cleaving other cellular substrates such as PARP and DFF, which in turn induce apoptosis. 

Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).

The SensoLyte® Caspase Profiling Kits and Substrate Sampler Kits contain a series of AFC or AMC-based peptide substrates, which yield blue fluorescence upon protease cleavage (AFC: Ex/Em=380 nm/500 nm; AMC: Ex/Em=354 nm/442 nm). They provide the best solution for profiling caspases or caspase inhibitors. In the Profiling Kits, the substrates are coated in 96-well plates. 

The SensoLyte® Homogeneous Caspase Assay Kits contain a fluorogenic substrate which, upon caspase cleavage, leads to fluorescence emission. 

A bi-functional assay buffer in the kits is optimized for cell lysis and measurement of the enzyme activity. These kits can assay caspase activity in cells grown in a 96-well plate or in larger plates or flasks. The kits are adaptable for high throughput screening of apoptosis inducers and inhibitors.

Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to development of diseases, such as cancer, organ failure and neurodegenerative diseases. Caspases are a large family of cysteinyl aspartate-specific proteases. They are activated in response to diverse cell death stimuli and ultimately dismantle a cell through restricted proteolysis of numerous cellular proteins, leading to apoptotic cell death.

The SensoLyte® 520 Generic Caspase Assay Kit provides a method for in situ detection of caspase activity in cells undergoing apoptosis using a fluorochrome inhibitor of caspase, FAM-VAD-FMK. This reagent is cell permeable and non-cytotoxic. When added to a population of cells, it enters cells and covalently binds to activated caspases-1, -3, -4, -5, -7, -8 and –9. Bound FAM-VAD-FMK is retained within the cell, while any unbound FAM-VAD-FMK diffuses out of the cells and is washed away. Signal in the cells containing the bound FAM-VAD-FMK can be evaluated with a fluorescence plate reader, fluorescence microscopy or flow cytometry.

DescriptionSize Reference USD Qty  
SensoLyte® AFC Caspase Profiling Kit *Fluorimetric* - 1 kit1 kit AS-71116 691.00Add to cart
SensoLyte® AFC Caspase Substrate Sampler Kit *Fluorimetric* - 1 kit1 kit AS-71117 552.00Add to cart
SensoLyte® AMC Caspase Profiling Kit *Fluorimetric* - 1 kit1 kit AS-71120 691.00Add to cart
SensoLyte® AMC Caspase Substrate Sampler Kit *Fluorimetric* - 1 kit1 kit AS-71121 552.00Add to cart
SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71114 300.00Add to cart
SensoLyte® Homogeneous AMC Caspase-3/7 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71118 300.00Add to cart
SensoLyte® Homogeneous Rh110 Caspase-3/7 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71141 300.00Add to cart


SensoLyte® AFC or AMC Caspase Profiling Kits contain a well-designed plate in which a series of AFC or AMC-based caspase substrates are coated with both positive and negative controls. 

SensoLyte® Caspase Substrate Sampler Kits contain a series of AFC or AMC-based peptide substrates. 

SensoLyte® Homogeneous AFC Caspase Assay Kits contain a fluorogenic substrate which emits fluorescence upon cleavage.


λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Caspase
SensoLyte® Caspase AFC Profiling Kit
Fluorimetric
380/500 50 μl n.a 2x 96-well plate coated with 8 different AFC-based caspase substrates (1-2-3/7-6-8-9) along with reference standard • AFC, fluorescence reference standard • Assay buffer • DTT • 10X Lysis Buffer Minimal 2 x 96-well plates
SensoLyte® Caspase AMC Profiling Kit
Fluorimetric
354/442 50 μl n.a

2x 96-well plate coated with 8 different AMC-based caspase substrates (1-1/4-3-3/7-6-8-9) along with reference standard
• AMC, fluorescence reference standard • Assay buffer • DTT
• 10X Lysis Buffer

Minimal 2 x 96-well plates
SensoLyte® AFC Caspase Substrate Sampler Kit
Fluorimetric
380/500 50 μl n.a 8 different AFC-based Caspase substrates (1-2-3/7-6-8-9) • AFC, fluorescence reference standard • Assay buffer • DTT • 10X Lysis Buffer Minimal 8 x100 assays
SensoLyte® AMC Caspase Substrate Sampler Kit
Fluorimetric
354/442 50 μl n.a 8 different AMC-based Caspase substrates (1-1/4-3-3/7-6-8-9)
• AMC, fluorescence reference standard • Assay buffer • DTT
• 10X Lysis Buffer
Minimal 8 x100 assays
SensoLyte® Homogenous AnaRed Caspase-3/7 Assay Kit
Fluorimetric
537/611 1 x 104-6 cells in 100 μl HTP Ac-DEVD-AnaRed™ Caspase-3/7 substrate • AnaRed™, fluorescence reference standard • caspase-3/7 inhibitor
(Ac-DEVD-CHO) • Assay buffer • DTT • 10X Lysis Buffer
Minimal 500
SensoLyte® Homogenous Rh110 Caspase-3/7 Assay Kit
Fluorimetric
496/520 1 x 103-6 cells in 100 μl HTP (Ac-DEVD)2-Rh110 Caspase-3/7 substrate • Rh110, fluorescence reference standard • caspase-3/7 inhibitor
(Ac-DEVD-CHO) • Assay buffer • DTT • 10X Lysis Buffer
Minimal 500
SensoLyte® Homogenous AFC Caspase-3/7 Assay Kit
Fluorimetric
380/500 1 x 103-6 cells in 100 μl HTP Ac-DEVD-AFC Caspase-3/7 substrate • AFC (7-amido-4-trifluoromethylcoumarin), fluorescence reference standard
• caspase-3/7 inhibitor (Ac-DEVD-CHO) • Assay buffer • DTT
• 10X Lysis Buffer
Minimal 500
SensoLyte® Homogenous AMC Caspase-3/7 Assay Kit
Fluorimetric
354/442 1 x 103-6 cells in 100 μl HTP Ac-DEVD-AMC Caspase-3/7 substrate • AMC (7-amino-4-methylcoumarin), fluorescence reference standard
• caspase-3/7 inhibitor (Ac-DEVD-CHO) • Assay buffer • DTT
• 10X Lysis Buffer
Minimal 500
SensoLyte® Homogenous AFC Caspase-8 Assay Kit
Fluorimetric
380/500 1 x 104-6 cells in 100 μl HTP Ac-IETD-AFC, caspase-8 substrate • AFC, fluorescence reference standard • Inhibitor of caspase-8, Ac-IETD-CHO • Assay Buffer (for cell lysis and activity measurements) • DTT Minimal 100 or 200

 

SensoLyte® 520 Generic Caspase Assay Kit *Fluorimetric*

  • Kit Size: 100 Assays
  • Optimized Performance: This kit is optimized for detecting generic caspase activity in cells
  • Enhanced Value: It provides ample reagents to perform 100 assays
  • High Speed: The cells can be stained and quantified within 2 hours
  • Assured Reliability: Detailed protocol and references are provided
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