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Cathepsin Assay Kits

iD Sensolyte® Assay Kits

Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. 

Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. It is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease. 

Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease. 

Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis, cancer, obesity and related diseases. 

Cathepsin G is the serine protease released by neutrophils upon their activation. Cathepsin G is currently being explored as a target for anti-inflammatory and anti-infective drugs. 

Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis. 

Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases. It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins. 

Cathepsin E is a non-lysosomal aspartic proteinase of the pepsin superfamily. It plays an important role in the protein degradation, the generation of bioactive proteins, and antigen processing. Recent studies have particularly suggested that Cathepsin E is important in host defense against cancer cells and invading microorganisms.

The SensoLyte® 390 Cathepsin D and E Activity Assay Kit use a Mca/Dnp FRET peptide for measurement of enzyme activity. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D/E, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330 nm/390 nm. 

The SensoLyte® 520 Cathepsin D, B, G, K, L and E Activity Assay Kits use a QXL™ 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is optimal for cathepsin activity. 

The SensoLyte® 520 Cathepsin S Activity Assay Kit uses a QXL™ 520/5-FAM based FRET peptide as substrate. 

The SensoLyte® 440 Cathepsin B and S Assay Kits provide a fluorogenic peptide for measurement of enzyme activity. This peptide releases the AMC (7-amino-4-methylcoumarin) fluorophore upon cathepsin cleavage and can be detected with excitation at 354 nm and emission at 442 nm. 

The SensoLyte® Rh110 Cathepsin G, K and L Assay Kits provide a convenient assay for high throughput screening of enzyme inhibitors or for continuous assay of enzyme activity utilizing a fluorogenic peptide. Upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=490/520 nm.

DescriptionSize Reference USD Qty  
SensoLyte® 390 Cathepsin D Assay Kit *Fluorimetric* - 1 kit1 kit AS-72098 413.00Add to cart
SensoLyte® 440 Cathepsin B Assay Kit *Fluorimetric* - 1 kit1 kit AS-72165 418.00Add to cart
SensoLyte® 440 Cathepsin S Assay Kit *Fluorimetric* - 1 kit1 kit AS-72100 413.00Add to cart
SensoLyte® 520 Cathepsin B Assay Kit *Fluorimetric* - 1 kit1 kit AS-72164 480.00Add to cart
SensoLyte® 520 Cathepsin D Assay Kit *Fluorimetric* - 1 kit1 kit AS-72170 480.00Add to cart
SensoLyte® 520 Cathepsin E Assay Kit *Fluorimetric* - 1 kit1 kit AS-72222 457.00Add to cart
SensoLyte® 520 Cathepsin G Assay Kit *Fluorimetric* - 1 kit1 kit AS-72185 480.00Add to cart
SensoLyte® 520 Cathepsin K Assay Kit *Fluorimetric* - 1 kit1 kit AS-72171 501.00Add to cart
SensoLyte® 520 Cathepsin L Assay Kit *Fluorimetric* - 1 kit1 kit AS-72218- 480.00Add to cart
SensoLyte® 520 Cathepsin S Assay Kit *Fluorimetric* - 1 kit1 kit AS-72099 480.00Add to cart
SensoLyte® Rh110 Cathepsin G Assay Kit *Fluorimetric* - 1 kit1 kit AS-72186 480.00Add to cart
SensoLyte® Rh110 Cathepsin K Assay Kit *Fluorimetric* - 1 kit1 kit AS-72152 501.00Add to cart
SensoLyte® Rh110 Cathepsin L Assay Kit *Fluorimetric* - 1 kit1 kit AS-72217 480.00Add to cart

Description

λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Cathepsin Assay Kit
SensoLyte® 520 Cathepsin S
Fluorimetric
490/520 5-50 μl 0.1 ng/well • Cathepsin S substrate: 5-FAM/QXL™ 520 FRET Peptide
• 5-FAM, fluorescence reference standard
• Cathepsin S, human spleen
• Assay Buffer
• Cathepsin S Inhibitor
• DTT
1 hour 100
SensoLyte® 440 Cathepsin S
Fluorimetric
354/442 5-50 μl 0.1 ng/well • AMC (7-amino-4-methylcoumarin) Cathepsin S substrate,
• AMC fluorescence reference standard
• Cathepsin S, human spleen
• Assay Buffer
• Cathepsin S Inhibitor
• DTT
1 hour 100
Sensolyte® 390  Cathepsin D
Fluorimetric
330/390 5-50 μl 0,15 µM • Cathepsin D substrate Mca/Dnp-based FRET peptide
• Mca Fluorescence reference standard
• Cathepsin D, bovine spleen
• Assay buffer
• Cathepsin D Inhibitor
• DTT

60 min 100
Sensolyte® 520
Cathepsin D
Fluorimetric
490/520 5-50 μl 0,078 µM • Cathepsin D substrate Mca/Dnp-based FRET peptide
• HiLyte Fluor™ 488 Fluorescence reference standard
• Cathepsin D, bovine spleen
• Assay buffer
• Cathepsin D Inhibitor
• DTT
60 min 100
Sensolyte® 520
Cathepsin B
Fluorimetric

490/520 5-50 μl 0,312µM • Cathepsin B substrate HiLyte Fluor™  488/QXL™520-based FRET peptide
• HiLyte Fluor™ 488 Fluorescence reference standard
• Cathepsin B, human liver
•Assay buffer
• Cathepsin B Inhibitor
• DTT
60 min 100
SensoLyte® 440 Cathepsin B
Fluorometric
354/442 5-50 μl 0,078 µM • Cathepsin B fluorogenic substrate
• AMC fluorescence reference standard
• Cathepsin B, human liver
• Assay buffer
• Cathepsin B Inhibitor
• DTT
60 min 100
Sensolyte® 520
Cathepsin G
Fluorimetric
490/520 5-50 μl 0,015 µM • Cathepsin G substrate HiLyte Fluor™ 488/QXL™520-based FRET peptide
• HiLyte Fluor™ 488 Fluorescence reference standard
• Cathepsin G, human neutrophil
• Assay buffer
• Cathepsin G Inhibitor
60 min 100
Sensolyte® Rh110
Cathepsin G
Fluorimetric
490/520 5-50 μl 0,23 µM • Rh110 Cathepsin G fluorogenic peptide substrate
• Rh110 Fluorescence reference standard
• Cathepsin G, human neutrophil
• Assay buffer
• Cathepsin G Inhibitor
60 min 100
Sensolyte® 520
Cathepsin K
Fluorimetric
490/520 5-50 μl 0,156 µM • Cathepsin K substrate HiLyte Fluor™ 488/QXL™520-based FRET peptide
• HiLyte Fluor™ 488 Fluorescence reference standard
• Procathepsin K, human recombinant
• Assay buffer
• Cathepsin K Activation buffer
• Cathepsin K Inhibitor
• DTT
60 min 100
Sensolyte® Rh110
Cathepsin K
Fluorimetric
490/520 5-50 μl 1 ng/well • Rh110 Cathepsin K fluorogenic peptide substrate
• Rh110 Fluorescence reference standard
• Procathepsin K, human recombinant
• Assay buffer
• Cathepsin K Activation buffer
• Cathepsin K Inhibitor
• DTT
60 min 100
Sensolyte® 520
Cathepsin L
Fluorimetric
490/520 5-50 μl 0,156 µM • Cathepsin L substrate HiLyte Fluor™ 488/QXL™520-based FRET peptide
• HiLyte Fluor™ 488 Fluorescence reference standard
• Cathepsin L, human recombinant
• Assay buffer
• Cathepsin L Inhibitor
• DTT
60 min 100
Sensolyte® Rh110
Cathepsin L
Fluorimetric
490/520 5-50 μl 0,1 µM • Rh110 Cathepsin L fluorogenic peptide substrate
• Rh110 Fluorescence reference standard
• Cathepsin L, human recombinant
• Assay buffer
• Cathepsin L Inhibitor
• DTT
60 min 100
SensoLyte® 390
Cathepsin E
Fluorimetric
330/390 5-50 μl 0,625 µM • Mca/Dnp, Cathepsin E substrate
• Mca Fluorescence reference standard
• Cathepsin E, human recombinant
• Assay buffer
• Pepstatin A
60 min 100
Sensolyte® 520
Cathepsin E
Fluorimetric
490/520 5-50 μl 0,156 µM • Cathepsin E substrate /HiLyte Fluor™ 488/QXL™ 520-based FRET peptide
• HiLyte Fluor™ 488 Fluorescence reference standard
• Cathepsin E, human recombinant
• Assay buffer
• Pepstatin A
60 min 100
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