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Cell Proliferation anc Cytotoxicity Assay Kits

iD Sensolyte® Assay Kits

We offer convenient assay kits for monitoring cell viability. Fluorescent dye-based assays for cell viability and cytotoxicity are reliable and easy to perform. Our DHL™ viability and cytotoxicity assay kits are ideal for the quantifying the proportion of live and dead cells in a mixed population. In contrast, proliferation assays are designed to monitor the growth rate of a cell population. 

Advancements include: 

  • Safety – non-radioactive, non-toxic 
  • Convenience – Ready-to-use reagents, no labeling required 
  • Accuracy – Correlate well with traditional isotope-based assays
DHL™ Cell Viability and Cytotoxicity Assay Kits use a sensitive fluorimetric indicator, resazurin, to measure the activity of lactate dehydrogenase (LDH) in cytoplasm (for live cells) or the medium (for dead cells). The measurement of LDH activity is a well-substantiated method for the quantification of viable cell numbers and the monitoring of cell proliferation. The presence of cytoplasmic LDH in the culture medium caused by the damage of cell membrane integrity is a measurable indicator of cell death and cytotoxicity.
 
The SensoLyte® Calcein Cell Viability Assay Kit provides a convenient tool to detect live cells. This kit can be used in proliferation, cytotoxicity, invasion, adhesion, migration and other cell-based assays. Calcein AM (calcein acetoxymethyl ester) is a non-fluorescent reagent that easily permeates cellular membrane. Once it permeates live cells, esterases remove the acetomethoxy group allowing calcein to bind to calcium, which results in a strong green fluorescence. The increase of fluorescence is proportional to the number of live cells. Inside dead cells, the non-fluorescent calcein AM remains intact due to the lack of active esterases. The fluorescent signal from the conversion of calcein AM to calcein can be monitored at Ex/Em=494nm /520 nm.

 

DescriptionSize Reference USD Qty  
SensoLyte® Calcein Cell Viability Assay Kit *Fluorimetric* - 1 kit1 kit AS-72126 272.00Add to cart
SensoLyte® Cell Cytotoxicity Assay Kit *Fluorimetric* - 1 kit1 kit AS-71302 259.00Add to cart
SensoLyte® Cell Cytotoxicity Assay Kit *Fluorimetric*, Large size - 1 kit1 kit AS-71303 924.00Add to cart
SensoLyte® Cell Viability and Proliferation Assay Kit *Fluorimetric* - 1 kit1 kit AS-71300 259.00Add to cart
SensoLyte® Cell Viability and Proliferation Assay Kit *Fluorimetric*, Large size - 1 kit1 kit AS-71301 601.00Add to cart

Benefits

  • Safety: Non-radioactive, non-toxic
  • Convenience: Ready-to-use reagents, no labeling required
  • Accuracy: Correlate well with traditional isotope-based assays

Description

λ
(nm)
Biological sample volume  Kit contains Assay time # of
assays in
96-well plate
Lactate dehydrogenase (LDH) Assay Kit
DHL™ Cell Viability and Proliferation Fluorimetric 560/590 upon
conversion
From 48 living cells up to 5X104 cells with a linear range (r2=0.99)

• Assay solution

2-24 h 2000 or 10000
DHL™ Cell Cytotoxicity
Fluorimetric
560/590 upon conversion From 100 dead cells up to 2.5X104 cells with a linear range (r2=0.95)

• Assay buffer A
• Assay buffer B
• Lysis solution
• Stop solution

10 min 500 or 5000
Calcein          
SensoLyte® Calcein Cell Viability
Fluorimetric
494/520
upon
conversion
2x102-4 cells • Calcein AM in anhydrous DMSO solution
• 10X Assay buffer
2-24 h 1000
The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

Please use your Login or Register Now to directly access your customized EOS interface.



You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
  delivery address product description and reference number
  invoice address quotation number
  telephone number  

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