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Custom Mouse Monoclonal Program

Monoclonal Antibodies

Mab development - anti-Peptide & anti-Protein


Eurogentec is pleased to provide you with Custom Monoclonal Antibodies. Standard development program lasts 16-19 weeks. However, a personalised procedure may be designed and adapted to your needs (accelerated program and a a large panel of additional services are available on request).

To start discussing your project and benefit from a personalised project proposal adapted to your needs, please contact us.

DescriptionSize Reference USD
Monoclonal antibody - Phase I - Immunisation - per mouse (minimum 4 mice)1 mouse AS-ACMC-IM 603.00
Monoclonal antibody - Phase II - Fusion - Eight 96 well plates8 plates AS-ACMC-FU08 2 998.00
Monoclonal antibody - Phase III - Analysis - 8 plate fusion  AS-ACMC-AN08S 2 746.00
Monoclonal antibody - Phase IV - Cloning and isotyping - 1 or 2 hybridoma  AS-ACMC-CIFH 3 020.00
Monoclonal service - IgG purification - max 100 mg antibody from 500 ml supernatant  AS-PURI-MCIGG500 On Request
Monoclonal service - IgM purif - From 100 ml supernatant, including QC by SDS-PAGE  AS-PURI-MCIGM100 On Request
Monoclonal service - IgM purification-From 500ml supernatant - including QC  AS-PURI-MCIGM500 On Request
Monoclonal service - mAb lyophilization  AS-ACMC-LY0100 On Request

Starting Material


To start immunisation programme please send us:
  • your purified protein of interest
  • OR your peptide sequence
  • OR your protein's accession number

Phase I: Immunization ~ 6 weeks


  • A minimum of four mice (6-8 weeks old) are used for immunization.
  • Preimmune serum is taken on day 0.
  • A total of four injections are administered (50 µg antigen per animal per injection).
    The first injection on day 0 with equal parts (v/v) of complete Freund's adjuvant and antigen.
    The following three boosts are carried out with equal parts (v/v) incomplete Freund's adjuvant and antigen on days 21 and 36 with an additional final boost prior to fusion.
  • Selection of the best responding on the basis of Customer's test results.
If the antigen has a low immunogenicity, it can be coupled to an appropriate protein carrier.

Phase II: Fusion/Hybridoma production~ 2-3 weeks


The splenic lymphocytes of the best responding animal will be fused with the cell line Sp2/O-Ag-14 using PEG (polyethylene glycol), and the resulting hybridomas will be seeded in eight 96-well plates in HAT medium.
Duration: 2-3 weeks

Phase III: Screening for positive hybridomas ~ 4-5 weeks


  • Screening for positive antibody producing hybridomas (ELISA against the target antigen)
  • Positive colonies are expanded and tested for antibody production
    On request, positive colonies can also be screened against other antigens for cross-reactivity or against a specific region of the antigen of interest
    Different screening techniques are available and can be used on request. Please contact us for more information
  • > 1 ml of positive supernatants (maximum 30) will be sent to customer for screening confirmation and additional tests.

Phase IV: Cloning and isotyping of positive hybridomas ~ 4-5 weeks

  • The specific hybridomas selected by the customer at the end of the Phase III are cloned by serial dilution.
  • Positive hybridomas are collected in vials
  • Monoclonal antibody isotype is determined
  • > 10 ml of supernatant from 1-2 positive clones and frozen hybridomas are delivered
The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

Please use your Login or Register Now to directly access your customized EOS interface.



You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
  delivery address product description and reference number
  invoice address quotation number
  telephone number  

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