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Custom siRNA

siRNA

The easiest and most efficient way to achieve RNAi is to use synthetic small interfering RNAs (siRNA). Standard siRNAs are duplexes of short mixed oligonucleotides. These are typically made of 19 RNA nucleotides with symmetric 2 nucleotides (usually DNA bases) 3’ overhangs.
 

DescriptionSize Reference USD  
siRNA Duplex RP-HPLC 3 nmol delivered - 1 duplex1 duplex SR-HP001-001 180.00Configure...
siRNA Duplex RP-HPLC 12 nmol delivered - 1 duplex1 duplex SR-HP001-004 239.00Configure...
siRNA Duplex RP-HPLC 40 nmol delivered - 1 duplex1 duplex SR-HP001-020 348.00Configure...
siRNA Duplex RP-HPLC 80 nmol delivered - 1 duplex1 duplex SR-HP001-100 468.00Configure...
siRNA Duplex RP-HPLC 2,5 µmol scale - 1 duplex1 duplex SR-HP001-M02 On RequestConfigure...
siRNA Duplex RP-HPLC 5 µmol scale - 1 duplex1 duplex SR-HP001-M05 On RequestConfigure...
siRNA Duplex RP-HPLC 10 µmol scale - 1 duplex1 duplex SR-HP001-M10 On RequestConfigure...
siRNA Duplex RP-HPLC 20 µmol scale - 1 duplex1 duplex SR-HP001-M20 On RequestConfigure...
siRNA Duplex IEX-HPLC 3 nmol delivered - 1 duplex1 duplex SR-IE001-001 180.00Configure...
siRNA Duplex IEX-HPLC 12 nmol delivered - 1 duplex1 duplex SR-IE001-004 239.00Configure...
siRNA Duplex IEX-HPLC 40 nmol delivered - 1 duplex1 duplex SR-IE001-020 348.00Configure...
siRNA Duplex IEX-HPLC 80 nmol delivered - 1 duplex1 duplex SR-IE001-100 468.00Configure...
siRNA Duplex IEX-HPLC 2,5 µmole scale - 1 duplex1 duplex SR-IE001-M02 On RequestConfigure...
siRNA Duplex IEX-HPLC 5 µmole scale - 1 duplex1 duplex SR-IE001-M05 On RequestConfigure...
siRNA Duplex IEX-HPLC 10 µmole scale - 1 duplex1 duplex SR-IE001-M10 On RequestConfigure...
siRNA Duplex IEX-HPLC 20 µmole scale - 1 duplex1 duplex SR-IE001-M20 On RequestConfigure...
siRNA Duplex SePOP 7 nmol delivered - 1 duplex1 duplex SR-NP001-001 132.00Configure...
siRNA Duplex SePOP 22 nmol delivered - 1 duplex1 duplex SR-NP001-004 174.00Configure...
siRNA Duplex SePOP 60 nmol delivered - 1 duplex1 duplex SR-NP001-020 246.00Configure...
siRNA Duplex SePOP 200 nmol delivered - 1 duplex1 duplex SR-NP001-100 330.00Configure...
Standard siRNA are made of 19 RNA bases + 2 DNA bases 3' overhang. Additional bases could be charged according to RNA list price

Delivered Quantity



Synthesis Scale Delivered Quantity (nmol)
  SePOP HPLC (RP/IEX)
10 nmol 7 3
40 nmol 22 12
200 nmol 60 40
1000 nmol 200 80


Quality Control

   MALDI-TOF Mass Spectrometry

Delivery times

   5 Working days

Packaging

   Annealed in solution or not annealed lyophilized

Shipping conditions

   Dry ice (solution) or Room temperature (lyophilized)

Storage conditions

  

The below table describes oligonucleotide stability and storage recommendations, in a nuclease free environment. For long-term high quality storage, prepare workable aliquots to avoid multiple freeze-thaw cycles (not more than 5).


Format Storage* Stability**
Dried RT 18 months
RNase-free Buffer (pH 7.5) -20°C 24 months


* Oligonucleotides and especially dye-labelled oligonucleotides should be protected from light for optimal stability. Tolerance -20°C ± 5°C.
** Please note that depending on sequence and modi?cations, the stability of the oligos may vary substantially versus the given values, which should therefore be considered as indicative. Although it is generally recommended to avoid freeze-thaw cycles, we did not observe negative effects in qPCR tests after 10 freeze-thaw cycles.

  • 2 complementary siRNA strands (19 to 25 RNA bases plus 2 x 3’-DNA overhang bases)
  • 10, 40, 200 or 1000 nmol synthesis scale (larger scales available on request)*
  • SePOP Desalted or IEX/RP-HPLC purified
  • 100 % MALDI-TOF Mass Spectrometry controlled
  • In order to ensure maximum stability and, if not annealed, all siRNAs are provided lyophilized in individual tubes.
  • Free annealing on request (shipped with free annealing buffer and RNase-free water).

Please note that each single siRNA strand can be ordered separately.

*Synthesis scale vs delivered quantity



Synthesis Scale Delivered Quantity (nmol)
  SePOP HPLC (RP/IEX)
10 nmol 7 3
40 nmol 22 12
200 nmol 60 40
1000 nmol 200 80


Legal notices

   For Research Use only

Order Forms

Literature & Resources

Product citations



NOVO C. L. et al., "A new role for histone deacetylase 5 in the maintenance of long telomeres", The FASEB Journal, doi: 10.1096/fj.12-224204, May 2013

PEIXOTO P. et al., "HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells", Cell Death and Differentiation, 19, 1239–1252; doi:10.1038/cdd.2012.3, February 2012

POTENTE M. et al., "11,12-Epoxyeicosatrienoic Acid-induced Inhibition of FOXO Factors Promotes Endothelial Proliferation by Down-Regulating p27Kip1", Journal of Biological Chemistry, vol. 278, n° 32, p. 29619-29625, May 2003


TUROWSKI P. et al., "Functional cdc25C Dual-Specificity Phosphatase Is Required for S-Phase Entry in Human Cells", Molecular Biology of the Cell, vol. 14, Issue 7, p. 2984-2998, July 2003


AVEROUST J. et al., "Induction of CHOP Expression by Amino Acid Limitation Requires Both ATF4 Expression ATF2 Phosphorylation", Journal of Biological Chemistry, vol. 279, n° 7, p. 5288-5297, February 2004


LAZRAK M. et al., "The bHLH TAL-1/SCL regulates endothelial cell migration and morphogenesis", Journal of Cell Science, 117, 1161-1171, February 2004


DUTTON A. et al., "Expression of the cellular FLICE-inhibitory protein (c-FLIP) protects Hodgkin's lymphoma cells from autonomous Fas-mediated death", PNAS, vol. 101, n° 17, p. 6611-6616, April 2004


VOURET-CRAVIARI V. et al., "ILK is required for the assembly of matrix-forming adhesions and capillary morphogenesis in endothelial cells", Journal of Cell Science, 117, p. 4559-4569, May 2004


YU L.-G. et al., "Protein Phosphatase 2A, a Negative Regulator of the ERK Signaling Pathway, Is Activated by Tyrosine Phosphorylation of Putative HLA Class II-associated Protein I (PHAPI)/pp32 in Response to the Antiproliferative Lectin, Jacalin", Journal of Biological Chemistry, vol. 279, n° 40, p. 41377-41383, October 2004


STEWART S. et al., "Epstein–Barr virus-encoded LMP2A regulates viral and cellular gene expression by modulation of the NF-B transcription factor pathway", PNAS, vol. 101, n° 44, 15730-15735, November 2004


LAIGLE A. et al., "The reduction of P-Glycoprotein Expression by small interfering RNAs is improved in exponentially growing cells", Oligonucleotides, 14:191-198, 2004


WÜNSCHE W. et al., "The activity of siRNA in Mammalian Cells is related to the kinetics of siRNA-target recognition in vitro: Mechanistic Implications", Journal of Molecular Biology, vol. 345, 203-209, 2005


LOUIS K. et al., "Tumor Cell-mediated Induction of the Stromal Factor Stromelysin-3 Requires Heterotypic Cell Contact-dependent Activation of Specific Protein Kinase C Isoforms", Journal of Biological Chemistry, vol. 280, n° 2, p. 1272-1283, January 2005

To order easily your siRNA sequences you can either download the following document (siRNA Email Order Form) or directly order your siRNA through our ordering web interface in the siRNA module.


The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

Please use your Login or Register Now to directly access your customized EOS interface.



You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
  delivery address product description and reference number
  invoice address quotation number
  telephone number  


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