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DJ-1 (PARK7)

Custom & Catalog Recombinant Proteins

DJ-1 (also known as PARK7, Parkinson disease protein 7) is a 189-amino acid protein that is ubiquitously expressed in many organs including brain, liver, kidney, pancreas, heart, and others.1-5 Although DJ-1 was originally discovered as a novel oncogene product, it was found to play several other roles in biological processes. These include regulation of RNA binding activity, fertility, anti-oxidative stress, and is linked to the early onset of Parkinson disease when mutated.1-5 Recently, it was demonstrated that DJ-1 can act as an atypical peroxiredoxin-like peroxidase5 and possesses protease activity.4

DescriptionSize Reference USD Qty  
DJ-1 (PARK7), human recombinant - 10 µg10 µg AS-72219-10 100.00Order Form
DJ-1 (PARK7), human recombinant - 100 µg100 µg AS-72219-100 300.00Order Form
DJ-1 (PARK7), human recombinant - 500 µg500 µg AS-72219-500 950.00Order Form
DJ-1 (PARK7), human recombinant, GST Tagged - 10 µg10 µg AS-72220-10 100.00Order Form
DJ-1 (PARK7), human recombinant, GST Tagged - 100 µg100 µg AS-72220-100 300.00Order Form


Size:

  10 µg, 100 µg, 500µg

Storage:

  The purified DJ-1 is supplied as sterile and frozen at 1 mg/ml in 50 mM Tris-Cl/150mM NaCl, pH=8.0. Store at 2-4 ºC for immediate use within 1-2 weeks or at –80 ºC for up to 12 months.
Avoid repeated freeze-thaw cycles.

DJ-1 (also known as PARK7, Parkinson disease protein 7) is a 189-amino acid protein that is ubiquitously expressed in many organs including brain, liver, kidney, pancreas, heart, and others.1-5 Although DJ-1 was originally discovered as a novel oncogene product, it was found to play several other roles in biological processes. These include regulation of RNA binding activity, fertility, anti-oxidative stress, and is linked to the early onset of Parkinson disease when mutated.1-5 Recently, it was demonstrated that DJ-1 can act as an atypical peroxiredoxin-like peroxidase5 and possesses protease activity.4

The sequence (Accession # NP_001116849) corresponding to the full length human DJ-1 protein along with N-terminal GST tag was expressed in E. coli. The recombinant DJ-1 protein was purified from bacterial lysate using GST affinity chromatography. GST tag was removed (AS-72219) or not (AS-72220). The molecular weight of the recombinant DJ-1 protein is 19.9 kDa and 47.3 kDa for the GST-DJ-1recombinant protein.


Figure 1. Human DJ-1 on SDS-PAGE. Purified DJ-1 was loaded onto a 10-20% Tris-HCl gel at 3 g/well and resolved at 200V for 40 min. 1=Uninduced cell lysate, 2=Induced cell lysate, 3=Purified and cleaved DJ-1 protein.
Figure 2. Western Immunoblot of DJ-1. Purified DJ-1 protein was probed with anti-DJ-1 specific antibody.
Figure 3. DJ-1 Proteolytic Activity. DJ-1 was loaded at 200 μg/well with 1mM DTT and assayed for proteolytic activity using Green Protease Assay Kit (AnaSpec Cat # 71124).


Figure 1. Human GST-DJ-1 on SDS-PAGE. Purified GST-DJ-1 was loaded onto a 10-20% Tris-HCl gel at 3 g/well and resolved at 200V for 40 min. 1=Uninduced cell lysate, 2=Induced cell lysate, 3=Purified GST- DJ-1 protein.
Figure 2. Western Immunoblot of DJ-1. Purified GST-DJ-1 protein was probed with anti-DJ-1 specific antibody.
Figure 3. GST-DJ-1 Proteolytic Activity. GST-DJ-1 was loaded at 200 μg/well with 1mM DTT and assayed for proteolytic activity using Green Protease Assay Kit (AnaSpec Cat. # 71124).


References:


  1. Huai, Q. et al. FEBS Letters 549, 171 (2003).
  2. Inden, M. et al. J Pharma Sci 117, 189 (2011).
  3. Kitamura, Y. et al. Mol Neurodegener 6, 1 (2011).
  4. Chen, J. et al. Hum Mol Genetics 19, 2395 (2010).
  5. Andres-Mateos, E., et al. Proc Nat Acad Sci 104, 14807 (2007).

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