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DPPIV Assay Kits

iD Sensolyte® Assay Kits

DPPIV is a widely distributed serine protease that cleaves two amino acids from small peptides containing alanine or proline in the second position of the N-terminus of the peptide. DPPIV activity is especially critical for inactivation of GLP-1, a potent antihyperglycemic hormone, inducing glucose-dependent stimulation of insulin secretion. DPPIV inhibitors provide a potent treatment for type 2 diabetes prolonging the activity of GLP-1 and improving insulin secretion and blood glucose control regulation. 

The SensoLyte® DPPIV Assay Kits provide a convenient method for high throughput screening of DPPIV inhibitors and for continuous assay of DPPIV activity utilizing a fluorogenic peptide substrate.

In the SensoLyte® Rh110 DPIV Assay Kit, upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=490/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from reaction components. 

In the SensoLyte® AMC DPPIV Assay Kit, DPPIV cleaves the substrate resulting in release of AMC (7-amino-4-methylcoumarin) fluorophore. Fluorescence can be monitored at excitation /emission= 354/442 nm.

DescriptionSize Reference USD Qty  
SensoLyte® AMC DPPIV Assay Kit *Fluorimetric* - 1 kit1 kit AS-72197- 296.00Add to cart
SensoLyte® Rh110 DPPIV Assay Kit *Fluorimetric* - 1 kit1 kit AS-72196- 358.00Add to cart

Description

  λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Cathepsin Assay Kit
Sensolyte® Rh110 DPPIV
Fluorimetric
490/520 5-50 μl 0,39 µM • DPPIV D substrate
• Rh110 fluorescence reference standard
• DPPIV, porcine kidney
• Assay buffer
• DPPIV Inhibitor
1 hour 100
SensoLyte® AMC DPPIV Assay Kit 354/442 5-50 μl 0,39 µM • DPPIV D substrate
• AMC fluorescence reference standard
• DPPIV, porcine kidney
• Assay buffer
• DPPIV Inhibitor
1 hour 100

Performance: These kits are optimized to detect DPPIV activity 

Enhanced Value: Enough reagents are provided to perform 100 assays in a 96-well format 

High Speed: The entire process can be completed in one hour 

Reliability: Detailed protocol and references are provided 

Storage: -80°C

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