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ECEs activity assay kit

iD Sensolyte® Assay Kits

The SensoLyte® 520 ECEs Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations.
The substrate provided in this kit was based on a beta amyloid fragment.
When active ECEs cleave the FRET substrate, it results in an increase of 5-FAM fluorescence, and is monitored at excitation/emission = 490/520 nm.
The long wavelength fluorescence of 5-FAM shows less interference from autofluorescence of components in biological samples and test compounds.
This assay can detect as low as 15.6 ng/mL active ECE-1 and 62.5 ng/mL active ECE-2.

DescriptionSize Reference USD Qty  
SensoLyte® 520 ECEs Activity Assay Kit *Fluorimetric* - 1 kit 1 kit AS-72243 501.00Add to cart

The SensoLyte® 520 ECEs Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. The substrate provided in this kit was based on a beta amyloid fragment. When active ECEs cleave the FRET substrate, it results in an increase of 5-FAM fluorescence, and is monitored at excitation/emission = 490/520 nm. The long wavelength fluorescence of 5-FAM shows less interference from autofluorescence of components in biological samples and test compounds. This assay can detect as low as 15.6 ng/mL active ECE-1 and 62.5 ng/mL active ECE-2.

ECEs (ECE-1/ECE-2) are known as rate-limiting enzymes for the formation of the vasoconstrictor peptide endothelin-1 (ET-1) from its precursor proendothelin-1. ECE-1 can also degrade amyloid ß fragments both in vitro and in vivo. Highly homologous and structurally comparable to ECE-1, ECE-2 was shown in homozygous knockout mice to have decreased Aß degradation and significant elevated Aß 1-40 and Aß 1-42 in the brain.

  • Industry's First available kit for ECE Activity
  • Long wavelength fluorimetric kit means lower background, higher sensitivity
  • Direct detection of both ECE-1 and ECE-2 activity

The kit contains:
  • 5-FAM/QXL® 520 ECEs substrate, Ex/Em=490/520 nm upon cleavage
  • Assay buffer
  • Human recombinant ECE-1
  • Inhibitor
  • Fluorescence reference standard for calibration
  • Detailed protocol
  • Kit size: 100 assays (96-well plate).

 

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