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Elastase Assay Kits

iD Sensolyte® Assay Kits

Elastases catalyze the hydrolysis of elastin, the chief constituent of elastic fibers that together with collagen determines the mechanical properties of connective tissue. Elastases have many other natural substrates, these include proteoglycans, collagens and fibronectin. Implicated in many diseases such as pulmonary emphysema, cystic fibrosis, infections, inflammation and atherosclerosis, elastases are considered important drug targets. 

The SensoLyte® Green Elastase Assay Kit is a FRET-based assay that detects elastase activity. The kit provides an elastin, natural substrate for elastases, labeled with 5-FAM fluorophore and QXL™520 quencher. Elastase-catalyzed hydrolysis yields brightly green fluorescence. Increase in fluorescence intensity is directly proportional to enzyme activity. The kit does not require any separation steps and can be used to continuously measure the kinetics of elastase. 

The SensoLyte® Rh110 Elastase Assay Kit detects elastase activity using a fluorogenic peptide substrate. Upon elastase cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=490/520 nm. The increase in fluorescence intensity is directly proportional to enzyme activity. The kit does not require any separation steps and can be used to continuously measure the kinetics of elastase. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from screening compounds.

DescriptionSize Reference USD Qty  
SensoLyte® Green Elastase Assay Kit *Fluorimetric* - 1 kit1 kit AS-72178 272.00Add to cart
SensoLyte® Rh110 Elastase Assay Kit *Fluorimetric* - 1 kit1 kit AS-72179 272.00Add to cart

Description

  λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Elastase Assay Kits
Sensolyte® Rh110 Elastase
Fluorimetric
490/520 5-50 μl 0,156 µM • Rh110 elastase substrate
• Rh110, fluorescence reference standard
• Elastase, porcine pancreas
• 2X Assay buffer
• Elastase inhibitor
1 hour 100
Sensolyte® Green
Elastase
Fluorimetric
490/520 5-50 μl 0,39 µM • 5-FAM/QXL™ 520 labeled elastin
• Elastase, porcine pancreas
• 2X Assay buffer
• Elastase inhibitor
1 hour 100

 

The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

Please use your Login or Register Now to directly access your customized EOS interface.



You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
  delivery address product description and reference number
  invoice address quotation number
  telephone number  

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