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Factor Xa Assay Kits

iD Sensolyte® Assay Kits

Factor Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits. FXa leads to blood clot formation by converting prothrombin to thrombin through the prothrombinase complex. FXa is generated from zymogen Factor X via the intrinsic and extrinsic pathways and is the rate-limiting step in the propagation of thrombin generation. In the presence of Ca2+ ions, FXa forms prothrombinase with factor Va on the phospholipid membrane of the activated platelets. FXa has emerged as an attractive target for drug discovery for thromboembolic diseases.

The SensoLyte® Factor Xa Assay Kits provide a convenient way for screening of FXa inhibitors or for the continuous assay of enzyme activity. The assays are performed in a convenient 96-well microplate format.

The SensoLyte® 520 Factor Xa Assay Kit uses a 5-FAM/QXL™ 520 fluorescence resonance energy transfer (FRET) peptide. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage into two separate fragments by Factor Xa, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission wavelengths = 490 nm/520 nm. With excellent fluorescence quantum yield and longer wavelength, 5-FAM shows less interference from autofluorescence of test compounds and cellular components.

The SensoLyte® Rh110 Factor Xa Assay Kit uses a fluorogenic substrate. Upon FXa protease cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore which has a bright green fluorescence that can be detected at excitation/emission=490 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components.

DescriptionSize Reference USD Qty  
SensoLyte® 520 Factor Xa Assay Kit *Fluorimetric* - 1 kit1 kit AS-72208 501.00Add to cart
SensoLyte® Rh110 Factor Xa Assay Kit *Fluorimetric* - 1 kit1 kit AS-72207 457.00Add to cart

Optimized Performance: These kits are optimized to detect Factor Xa enzyme activity

Enhanced Value: They provide ample reagents to perform 100 assays in a 96-well plate format

High Speed: The entire process can be completed in one hour

Assured Reliability: Detailed protocol and references are provided

The kit contains:
  • Factor Xa substrate: Rh110 labeled (Ex/Em=490/520 nm upon cleavage) or 5-FAM/QXL™-520 labeled (Ex/Em=490/520 nm upon cleavage)
  • Fluorescence reference standard: Rh110 (Ex/Em=490/520 nm) or 5-FAM (Ex/Em=490/520 nm)
  • Purified Bovine Factor Xa
  • Assay Buffer
  • Factor Xa Inhibitor

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