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Generic protease assay kits

iD Sensolyte® Assay Kits

The SensoLyte® Green and Red Protease Assay Kits use a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green or red fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm or Ex/Em=546/575nm ). 

The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases.

DescriptionSize Reference USD Qty  
SensoLyte® Green Protease Assay Kit *Fluorimetric* - 1 kit1 kit AS-71124 272.00Add to cart
SensoLyte® Red Protease Assay Kit *Fluorimetric* - 1 kit1 kit AS-71140 272.00Add to cart


The SensoLyte® Green Protease Assay Kit uses casein that is heavily labeled with HiLyte FluorTM 488, a pH-insensitive green fluorophore, resulting in almost total quenching of its fluorescence. Proteolytic cleavage of this quenched casein-HiLyte FluorTM 488 conjugate yields brightly green fluorescence, which can be continuously monitored at excitation/emission= 488 nm/520 nm. The increase in fluorescence intensity is directly proportional to protease activity. This kit does not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases in acidic and basic buffer.

The SensoLyte® Red Protease Assay Kit uses casein heavily labeled with the red fluorophore 5(6)-TAMRA, giving upon cleavage a bright red fluorescence which can be continuously monitored at excitation/emission= 546 nm/575 nm.


Benefits

  • Optimized Sensitivity
    HiLyte™ Fluor 488 is a pH-insensitive green fluorophore
    5(6) TAMRA is a red fluorophore less interfered by the autofluorescence of sample compounds

Description

λ
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Protease assay kit
SensoLyte® Green Protease Assay Kit Fluorimetric 488/520 50 µl Used for ELISA

• Protease substrate HiLyte Fluor TM 488 -labeled casein
• Trypsin
• 2X Assay buffer

30-60 min 500
SensoLyte® Red Protease Assay Kit Fluorimetric 546/575 50 µl Used for ELISA

• Protease substrate 5(6) TAMRA -labeled casein
• Trypsin
• 2X Assay buffer

30-60 min 500

 


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You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
  delivery address product description and reference number
  invoice address quotation number
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