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Glutaminyl Cyclase Assay Kit

iD Sensolyte® Assay Kits

Many biologically active peptides and proteins have pyroglutamate (pGlu) as their first amino acid. This modification protects them from degradation by amino peptidases and in many cases determines functionality of protein or peptide. However, pyroglutamate modification sometimes may lead to negative side effects. For example, pGlu modified beta-amyloid peptides (pGlu-Aβ) aggregate much faster than non-pyroglutamic ones and form toxic oligomers that may damage neurons. Recent studies linked pGlu-Aβ to Alzheimer’s Disease because it was identified as a major component of amyloid plaques.

pGlu can be formed when glutamine or glutamic acid spontaneously converts into pyroglutamate. However, cyclic reaction rate is dramatically increased in the presence of Glutaminyl Cyclase (QC), also known as Glutaminyl-peptide Cyclotransferase (QPCT). Human Glutaminyl Cyclase has been isolated from nerve tissue, and detected in cerebrospinal fluid and saliva.

The SensoLyte® Green Glutaminyl Cyclase Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring enzyme activity from various sources using a green fluorescence substrate. During the first step, substrate is incubated with Glutaminyl Cyclase or enzyme-containing samples and converted into the pyroglutamate form. Second, glutaminyl cyclase developer is added to remove pGlu residue and generate the green fluorescence (λexc/em = 490/520 nm), which is proportional to the enzyme activity. This kit is ideal for high throughput screening (HTS) of glutaminyl cyclase activators and inhibitors. The long wavelength of the green fluorophore is less interfered by autofluorescence of cell components and test compounds. Assay sensitivity is 1.5 ng/mL of active enzyme.

DescriptionSize Reference USD Qty  
SensoLyte Green Glutaminyl Cyclase Activity Assay Kit "Fluometric" - 1 Kit1 kit AS-72230 561.00Add to cart

Optimized Performance: This kit is optimized to detect Glutaminyl Cyclase activity

Enhanced Value: It provides enough reagents to perform 100 assays in a 96-well format

High Speed: The entire process can be completed in two hours

Assured Reliability: Detailed protocol and references are provided

The kit contains:
  • Glutaminyl Cyclase Green Substrate
  • Pyroglu Fluorescent Substrate Standard
  • Glutaminyl Cyclase, Human Recombinant
  • Glutaminyl Cyclase Developer
  • Assay Buffer
  • Inhibitor of Glutaminyl Cyclase (1-Benzyl-Imidazole)
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