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Glutathione/Glutathione-S-Transferase Assay Kits

iD Sensolyte® Assay Kits

Glutathione (GSH) is an important antioxidant molecule that functions as a cofactor for a variety of enzymes and is involved in various biological processes. Total GSH includes reduced GSH and oxidized GSH (GSSG). The concentrations of GSH and GSSG and their molar ratio can be used for indicating the oxidative stress status. 

Glutathione S-Transferase (GST) enzymes, a family of isozymes plays a crucial role in body defense mechanisms against toxins and carcinogens. GSTs carry out their functions mostly by mediating their conjugation with reduced glutathione on substrates such as pharmaceutical and environmental pollutants. 

The SensoLyte® Total GSH Quantitation Assay Kit utilizes the widely used Elmann’s reagent and an optimized enzymatic recycling method for the quantification of total glutathione. Glutathione reductase converts oxidized glutathione to reduced glutathione. Reduced GSH reacts with Ellman’s Reagent [5, 5’-dithiobis (2-nitrobenzoic acid)] and generates 2-nitro-5-thiobenzoic acid (TNB) producing a yellow color that can be detected at absorbance 405-415 nm. The intensity of the color produced is proportional to GSH concentration. The kit also provides 5-sulfosalicylic acid for protein removal from biological samples. 

The SensoLyte® 520 Total GSH Quantitation Assay Kit utilizes an optimized enzymatic recycling method for the quantification of glutathione. Glutathione reductase converts oxidized glutathione to reduced glutathione. Upon reaction with reduced GSH, the novel proprietary thiol detection reagent supplied in the kit generates fluorescence which can be easily detected at excitation/emission =490/520 nm. The assay has a linear range of 0.2-100 uM GSH. The kit also provides 5-sulfosalicylic acid for protein removal from biological samples. 

The SensoLyte® Glutathione Cellular Assay Kit utilizes an optimized enzymatic method for determination of the glutathione levels in cell or tissue extracts. The substrate provided in the kit can selectively react with GSH in the reaction catalyzed by glutathione S-transferase (GST) and form a fluorescent glutathione adduct. The resulting blue fluorescence can be monitored at excitation /emission= 390 nm/480 nm. The assay has a linear range of 0-100mM of GSH. This kit does not require the usage of deproteination for biological samples. 

The SensoLyte® GST Activity Assay Kit utilizes an optimized method for detection of enzyme activity in biological samples. The kit provides substrate for GST enzyme, which upon reaction with reduced glutathione yields a fluorescent glutathione adduct. This produces bright blue fluorescence that can be monitored at excitation/emission =390 nm/480 nm.

DescriptionSize Reference USD Qty  
SensoLyte® 520 Total GSH kit *Fluorimetric* - 1 kit1 kit AS-72154 413.00Add to cart
SensoLyte® Glutathione Cellular Assay Kit *Fluorimetric* - 1 kit1 kit AS-72158 296.00Add to cart
SensoLyte® GST Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72157 296.00Add to cart
SensoLyte® Total GSH assay Kit *Colorimetric* - 1 kit1 kit AS-72153 413.00Add to cart

Description

λ
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
SensoLyte® 520 Total GSH Assay
Fluorimetric
Ex/Em
490/520
1-10 µl 0.2 μM with
a linear range up to 100 μM
• Fluorimetric Thiol Detection Reagent
• Reduced Glutathione Standard Stock Solution
• GSH Reductase
• NADPH
• Assay Buffer
• 5-Sulfosalicylic Acid (SSA)
30 min 100
SensoLyte® Total GSH Assay
Colorimetric
Abs
415
1-10 µl 1.5 μM with
a linear range up to 100 μM
• Thiol Detection Reagent (Ellman’s Reagent)
• Reduced Glutathione Standard Stock Solution
• GSH Reductase
• NADPH
• Assay Buffer
• 5-Sulfosalicylic Acid (SSA)
30 min 500
SensoLyte® Glutathione Cellular Assay Kit
Fluorimetric
Ex/Em
390/480
1 – 40 µl 15.6 µM with a linear range up to 500 µM • GSH detection reagent
• Reduced Glutathione standard
• Assay Buffer
• GST enzyme
• DMSO
60 min 100
SensoLyte® GST Activity Assay Kit
Fluorimetric
Ex/Em
390/480
1 – 50 µl 15 mU/ml with a linear range up to 500 mU/ml • GST Substrate
• GST Standard
• Assay buffer
• Reduced Glutathione
• DMSO
60 min 100
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