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iD Blue Ladder Revealer

iD Key Solution – Protein identification tools



The iD BlueLadder Revealer is a mouse monoclonal antibody targeting the blue dye of prestained proteins from MW ladders. This antibody can be used to visualize the marker blue bands throughout the whole process of western blotting from gels to membranes and up to X-Ray films. This makes the positioning of the marker on the blot very easy and highly accurate.

The antibody is provided alone or coupled to horse radish peroxidase (HRP).

DescriptionSize Reference USD Qty  
iD BlueLadder Revealer - 50 µl50 µl ID-MWLRV1-050 95.00Configure
iD BlueLadder Revealer - HRP - 50 µl50 µl ID-MWLRV1-H50 100.00Configure

The iD BlueLadder Revealer is a mouse monoclonal antibody targeting the blue dye of prestained proteins from MW ladders. This antibody can be used to visualize the marker blue bands throughout the whole process of western blotting from gels to membranes and up to X-Ray films. This makes the positioning of the marker on the blot very easy and highly accurate.

The antibody is provided alone or coupled to horse radish peroxidase (HRP).


Specificity

This antibody is specific for the blue dye used in prestained protein MW markers. It does not react with human, mouse, rat, chicken, hamster, monkey, yeast and E. coli proteins.


Format

Protein A purified monoclonal antibody.

  • iD BlueLadder Revealer is delivered in PBS containing 0.05% azide
  • iD BlueLadder Revealer-HRP is delivered in a proprietary buffer for HRP stabilization

Storage

Store at -20°C
Avoid multiple freeze-thaw cycles


Application

Western Blotting: 1/1000 – 1/5000 suggested dilution


Instruction for Western Blotting application

  1. Run a SDS-PAGE with a blue pre-stained molecular weight marker and transfer the proteins to a membrane
  2. Block the membrane using an appropriate blocking solution e.g. iD Blocking Kit (ID-WCBLO1-001; Eurogentec)
  3. Incubate the membrane with a mix of primary antibodies containing:

    • The primary antibody specific to your protein (for dilution, please refer to the instructions from the manufacturer);
    • The iD BlueLadder Revealer (recommended dilution : 1/2000)

    Note: If the primary antibody specific to your protein is HRP-conjugated, use the iD BlueLadder Revealer-HRP.

  4. If the primary antibodies are not HRP-conjugated, incubate the membrane with secondary antibodies coupled to HRP (or any other conjugate of your choice). An anti-mouse secondary antibody is to be used to detect the iD BlueLadder Revealer (murine primary antibody).
  5. Perform the detection according to the conjugate selected

  6. Note: if HRP is used, a colorimetric or chemiluminescent detection can be performed using respectively the iD TMB Substrate Kit (ID-SUTMB1) or iD HRP Substrate Kit (ID-SUHRP1) (Eurogentec).

The iD BlueLadder Revealer and the iD BlueLadder Revealer-HRP are compatible with the iD Western 1H Detection Kits (Eurogentec).

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