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Insulin Degrading Enzyme (IDE) Assay Kit

iD Sensolyte® Assay Kits

Insulin degrading enzyme (IDE), an extremely conserved Zn2+ metalloendopeptidase, belongs to the M16A family of metallopeptidases. IDE is expressed in almost all tissues and distributed in the cytosol. With great capability to degrade various peptides such as IGF-II, TGF-α, glucagon, amylin, calcitonin, ß-amyloid and amyloid precursor protein (APP), IDE is considered a physiological and pathological relevant enzyme. Growing evidences have indicated that IDE may be involved in the pathogenesis of Alzheimer’s disease and diabetes mellitus type 2.

The SensoLyte® 520 IDE Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. The unique FRET substrate was derived from an APP sequence designed to reduce cross reactivity with Neprilysin, ADAM10, TACE, BACE-1, and BACE-2. When active IDE cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence, which is monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.8 ng/mL active IDE.

DescriptionSize Reference USD Qty  
SensoLyte® 520 IDE Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72231 501.00Add to cart

Optimized Performance: This kit is optimized to detect IDE activity

Enhanced Value: Ample reagents to perform 100 assays in a 96-well format

High Speed: The entire process can be completed in one hour

Assured Reliability: Detailed protocol and references are provided

The kit contains:
  • 5-FAM/QXL520® IDE substrate, Ex/Em=490/520 nm upon cleavage
  • Assay buffer
  • Recombinant human IDE
  • Inhibitor
  • Fluorescence reference standard for calibration
  • A detailed protocol
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