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Universal Exogenous qPCR Positive Control

Quantitative PCR (qPCR) Reagents

Real-Time PCR assays are prone to inhibition by various substances found in many samples (clinical, soil, plant…). Carryover of reagents used for the isolation of nucleic acids can also inhibit amplification reactions. Other causes of false-negative results include target nucleic acid degradation, sample processing errors and thermocycler malfunction. 


Benefits
Specific
- Does not interfere with target amplification
- Avoid amplification of endogenous genes
Sensitive
- Compatible with low copy templates
- Available with dark quencher for maximal signal-to-noise ratio
Designed for multiplex assays
- Detected in the Yakima Yellow® (VIC®/JOE equivalent) or Cy®5 channel

Eurogentec’s Universal Exogenous qPCR positive Controls provide an accurate way to assess the integrity of your nucleic acid extraction and amplification assay.

IPC are labelled to Yakima Yellow or Cy®5. Custom labelling is available on request.

Alternatively fluorescent dye-labelled controls can be used as positive controls in digital PCR (dPCR) applications.

 

DescriptionSize Reference USD Qty  
qPCR Internal Positive Control Cy5-QXL670 - 200 rxn200 rxn RT-IPCC-Q02 276.00Add to cart
qPCR Internal Positive Control (IPC) DNA template - 1000 rxn1000 rxn RT-IPCD-1000 427.00Add to cart
qPCR Internal Positive Control (IPC) DNA template - 200 rxn200 rxn RT-IPCD-200 106.80Add to cart
qPCR Internal Positive Control (IPC) Yakima Yellow®-BHQ-1 - 200 rxn (50 µl)200 rxn (50 µl) RT-IPCY-B02 276.00Add to cart
qPCR Internal Positive Control (IPC) Yakima Yellow®-BHQ-1 - 1000 rxn (50 µl)1000 rxn (50 µl) RT-IPCY-B10 1 236.00Add to cart
qPCR Internal Positive Control (IPC) Yakima Yellow®-TAMRA - 200 rxn (50 µl)200 rxn (50 µl) RT-IPCY-T02 276.00Add to cart
qPCR Internal Positive Control (IPC) Yakima Yellow®-TAMRA - 1000 rxn (50 µl)1000 rxn (50 µl) RT-IPCY-T10 1 236.00Add to cart

Delivery times

   2 WD if ordered before Thursday

Shipping conditions

   Dry ice

Storage conditions


For long-term storage, the Universal Exogenous qPCR Positive Control should be kept in the dark, at -20 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 1 year. 
For short-term storage, the Universal Exogenous qPCR Positive Control can be kept in the dark, at  4 °C to 6 °C for one month. 
The Universal Exogenous qPCR Positive Control should be protected from light whenever possible to avoid bleaching of the probe.

Eurogentec’s Universal Exogenous qPCR Positive Control for TaqMan® Assays is an optimised Taqman control that was designed to distinguish true target negatives from false negatives due to PCR inhibition, incorrect pipetting or cycling parameters.

  • The optimised control can be spiked into samples before extraction (SPC) or before qPCR Assay (IPC) without compromising amplification efficiency of the target sequence.
  • The optimised control doesn't match with any sequence routinely found in a lab. 
  • The optimised control is detected using a Yakima-Yellow® (VIC® equivalent) or a Cy®5-labelled probe and the target template is detected using a FAM-labeled probe.
  • Avoid amplification of endogenous genes.

High quality result achieved in 42' with FAST qPCR MasterMix Low ROX on an ABI Prism® 7500 FAST cycler using a ten times serial dilution of 18S cDNA (blue lines; detected in the FAM channel). (A) Assay performed in multiplex with the internal Positive Control (orange lines;YY-BHQ-1® version, detected in the VIC®/JOE channel). (B) Assay performed in singleplex, without internal Positive Control.

Note: Alternatively, the Universal Exogenous qPCR Positive Control may be used in standardised conditions as extraction yield calibrator, template quality sensor or inter-run calibrator.

  • A given quantity of control can be spiked into samples before extraction. A relative (directly comparing samples) or an absolute (using a dilution curve of the control) quantification is performed after extraction to normalize the extraction yields of the samples.
  • Quantitative results of the spiked control within the template or within a reference buffer (pure water, reference template…) are compared in order to reject templates where PCR inhibition is high (low quality).
  • Add a dilution series of the optimised control on each plate and use it to normalize PCR efficiencies between plates (also for cycler to cycler data normalization).

Literature & Resources

TDS

Download the Universal exogenous qPCR Positive Control Technical Data Sheets now:

MSDS

Download the qPCR Positive Control MSDS:

Certificate of analysis


To obtain the Certificate of Analysis corresponding to your qPCR kit of interest please contact us via email and display the reference code of the qPCR kit and the batch number. Contact us

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