Select your products and services :
Select your country :
Americas
Europe
Asia and South Pacific
Middle East and Africa

Update your choices | BU : Life Science | Country : USA

Kallikrein assay kit

iD Sensolyte® Assay Kits

 

Human plasma kallikrein, a serine protease, is first synthesized as an inactive plasma prekallikrein in liver and circulates in the plasma. Once activated by activated coagulation factor XII (FXIIa), plasma kallikrein releases bradykinin (an inflammatory mediator) from high molecular weight kininogen (HMWK). Plasma kallikrein is involved in several physiological and pathological processes such as blood coagulation, the classical complement cascade pathway and the activation of the alternative complement pathway.1, 2-5 Plasma kallikrein also plays a role in the induction of elastase release from neutrophils and conversion of prourokinase to urokinase in fibrinolysis.6-9

The SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit employs a substrate containing rhodamine 110 fluorophore (Rh110). Plasma kallikrein cleaves this Rh110 substrates to release a bright green fluorescent product that can be detected at 520 nm (Ex/Em: 496/520 nm).

Benefits:

  • Rh110 provides greater sensitivity and less interference from other reaction component
  • Detection of as low as 1ng/ml active plasma kallikrein
  • Detection of enzyme activity in purified enzyme preparations or biological samples. Can also be applied for compound screening

DescriptionSize Reference USD Qty  
SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72255 501.00Add to cart

1. Bryant, J.W. et al, Cardiovasc Hematol Agents Med Chem 7, 234 (2009)
2. Toossi, Z. et al. Proc Natl Acad Sci U S A 89, 11969 (1992)
3. Ghebrehiwet, B. et al. J Exp Med 153, 665 (1981)
4. Qin, X.P. et al. Clin Exp Pharmacol Physiol 36, 319 (2009)
5. Phipps, J.A. et al. Hypertension 53, 175 (2009)
6. DiScipio, R.G. Immunology 45, 587 (1982)
7. Kaplan, A.P. et al. J Exp Med 135, 81 (1972)
8. Wachtfogel, Y.T. et al. J Clin Invest 72, 1672 (1983)
9. Ichinose, A. et al, J Biol Chem 261, 3486 (1986)

 

 

Figure 1. Inhibition of Plasma Kallikrein activity by PKSI-527 as measured with SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit

Inhibition of Plasma Kallikrein activity

Figure 2. Kallikrein activity present in plasma samples was detected by SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit.

Kallikrein activity present in plasma

Figure 3. Plasma kallikrein activity detection in both human plasma and human prekallikrein depleted plasma with the use of SensoLyte® Rh110 Plasma Kallikrein Acitivty Assay

Plasma kallikrein activity detection
The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

Please use your Login or Register Now to directly access your customized EOS interface.



You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
  delivery address product description and reference number
  invoice address quotation number
  telephone number  

We use cookies to ensure the best experience on our website. By continuing to use this website, you consent to our cookie policy.