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Matriptase Assay Kit

iD Sensolyte® Assay Kits

Matriptase is a trypsin-like protease from the family of type II transmembrane serine proteases. Its actions can result in protein processing, activation or degradation.

The SensoLyte® Rh110 Matriptase Assay Kit provides a convenient assay for screening of enzyme inhibitors and activators or for continuous assay of enzyme activity using a fluorogenic substrate.

Optimized Performance: This kit is optimized to detect Matriptase activity
Enhanced Value: Ample reagents to perform 100 assays in a 96-well format
High Speed: The entire process can be completed in one hour
Assured Reliability: Detailed protocol and references are provided


DescriptionSize Reference USD Qty  
SensoLyte® Rh110 Matriptase Activity Assay Kit *Fluorimetric* - 1 kit 1 kit AS-72241 457.00Add to cart


Other Materials Required (but not provided)

  • 96-well microplate: Black, flat bottom 96-well plate with non-binding surface
  • Fluorescence microplate reader: Capable of detecting emission at 520 nm with excitation at 490 nm

Storage and Handling

  • Store all kit components at -20°C, except for Component C
  • Store Component C at -80°C
  • Protect Components A and B from light and moisture
  • Component D can be stored at room temperature for convenience

Matriptase (also known as MT-SP1, ST14, TADG-15 and epithin) is a trypsin-like protease from the family of type II transmembrane serine proteases. The spectrum of known matriptase substrates includes extracellular matrix proteins, cell adhesion molecules, ion channels, growth-factor-like proteins and other proteases. Its actions can result in protein processing, activation or degradation. Matriptase is widely expressed in virtually all epithelium and is specifically found in tumors of epithelial origin. Matriptase has been implicated in carcinogenesis including ovarian, prostate and cervical cancers and is considered a drug target for developing cancer therapeutic agent. 1, 2, 3

The SensoLyte® Rh110 Matriptase Assay Kit provides a convenient assay for screening of enzyme inhibitors and activators or for continuous assay of enzyme activity using a fluorogenic substrate. Upon matriptase cleavage, this substrate generates the Rh110 (Rhodamine 110) fluorophore which has a bright green fluorescence and can be detected at excitation/emission=496 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components .This assay can detect as low as 0.15 ng/mL active matriptase.

References

  • 1. Kimura T. et.al., J Biol Chem 288, 11 (2013): 7968-7977
  • 2. Galas M-C., et.al., J Biol Chem 281, 28 (2006): 19296-10394
  • 3. Moore J.D., et.al., Biorg and Med Chem Let 23 (2013): 4283-4291
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