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Meprin Activity Kits

iD Sensolyte® Assay Kits

Meprins are multimeric zinc proteases (members of the astacin family) related phylogenetically to MMPs and ADAMs. They can either be membrane-bound or secreted, and are highly expressed in the intestine and kidney. They play roles in connective tissue formation (and tissue differentiation), angiogenesis, cancer, immunology and neuroscience.

Meprin α can cleave extracellular matrix proteins (pro-collagen I, fibronectin, osteopontin, SPARC), growth factors (VEGF-A, CTGF) and proteinases (MMP-1). Evidence has suggested that Meprin α is associated with cancer proliferation, cancer migration and invasion. The FRET substrate (Ex/Em=490/520 nm upon cleavage) included in iD SensoLyte® 520 Meprin α Activity Assay Kit was designed to detect Meprin α activity and reduce the cross reactivity with Meprin β, ADAM10, α-secretase, BACE-2, ECEs and TACE.

Meprin β, is a type I transmembrane metalloprotease. Protein expression had been identified in multiple tissues and cell types. Though the biological function of Meprin β is not fully understood, Human Meprin β was found to specifically process APP in vivo, which may contribute to Alzheimer’s disease. The SensoLyte® 520 Meprin β Activity Assay Kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The unique FRET substrate was derived from APP sequence designed to reduce the cross reactivity with Meprin α, ADAM10, α-secretase, BACE-2, IDE and ECEs, Neprilysin and TACE.


Meprin α - 100 assays (96-well plate)

The FRET substrate included in SensoLyte® 520 Meprin α Activity Assay Kit was designed to detect Meprin α activity and reduce the cross reactivity with Meprin β, ADAM10, α-secretase, BACE-2, ECEs and TACE. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. Active Meprin ? cleaves the FRET substrate, resulting in an increase of 5-FAM fluorescence, monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.1 ng/mL active Meprin α.

The kit contains:

  • 5-FAM/QXL™ 520 Meprin α substrate, Ex/Em=490/520 nm upon cleavage
  • Assay buffer
  • Recombinant Human Meprin α
  • Meprin α activation buffer
  • Meprin α stop solution
  • Inhibitor
  • Fluorescence reference standard for calibration
  • A detailed protocol

Inhibition of Meprin alpha activity
Figure 1. Inhibition of Meprin α activity by Actinonin as measured with SensoLyte® 520 Meprin α Activity Assay Kit.

Meprin β - 100 assays (96-well plate)

The SensoLyte® 520 Meprin β Activity Assay Kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The unique FRET substrate was derived from APP sequence designed to reduce the cross reactivity with Meprin α, ADAM10, α-secretase, BACE-2, IDE and ECEs, Neprilysin and TACE. Active Meprin β cleaves the FRET substrate resulting in an increase of 5-FAM fluorescence, that can be monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.048 ng/mL active Meprin β.

The kit contains:

  • 5-FAM/QXL™ 520 Meprin β substrate, Ex/Em=490/520 nm upon cleavage
  • Assay buffer
  • Recombinant Human Meprin β
  • Meprin β activation buffer
  • Meprin β stop solution
  • Inhibitor
  • Fluorescence reference standard for calibration
  • A detailed protocol

Inhibition of Meprin beta activity
Figure 2. Inhibition of Meprin β activity by Actinonin as measured with SensoLyte® 520 Meprin β Activity Assay Kit.
The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
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All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

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