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Minimum guaranteed yields

Genomic oligonucleotides - Tube format

There is often some confusion surrounding the issues of synthesis scale and minimum guaranteed yield in custom oligonucleotide manufacturing:

  • The synthesis scale refers to the amount of raw material used to start the synthesis of oligonucleotides. 
  • The yield corresponds to the amount of final product recovered at the end of the synthesis and purification processes.

The length, the sequence, the type and number of modifications and a purification, strongly influence the amount of synthetized material and proportion of full length oligonucleotides. 

Based on that, Eurogentec defined a minimum guaranteed yield nmoles for all product categories (see table below). The applied minimum guaranteed yield is the minimum Guaranteed Yield in nmol. In many cases the minimum guaranteed yield represent only a reference because the delivered quantities are significantly higher.


Table: (1) Between 5 and 59 bases length single-modified Oligonucleotides. Eurogentec does not provide minimum guaranteed yield for modified Oligonucleotides greater than 59 bases. Post-synthesis modifications (not compatible with SePOP and RP-Cartridge•GoldTM purification) may yield 50% less than the above stated values. A lower yield may result from poly-modifications and/or strong secondary structures; (2) Double-Dye probes only result from the combination of a 5’ fluorescent dye and a 3’ quencher. Postsynthesis modifications may yield 50% less than the above stated values (see list below). (3) Except for Oligonucleotides with GC-rich regions; (4) Only available for Double-Dye FAM-TAMRA 10 nmol and FAM-BHQ1® 10 nmol ; (5) Non-modified siRNA's only include 3' dTdT overhang. For more information, please contact our Oligo Centre at oligo@eurogentec.com

List of the post-synthesis modifications:
> 5' Alexa Fluor® (350, 430, 488, 500, 514, 532, 546, 555, 568, 594, 610, 633, 647, 660, 680, 700 and 750)
> 5' ATTO (390, 425, 465, 488, 495, 520, 532, 550, 565, 590, 594, 610, 620, 633, 635, 647N, 655, 680, 700, 725 and 740)
> 5' BODIPY® (530/550, FL and TR)
> 3', 5' and dT Cascade Blue®
> 3' and dT Cy® (3, 3.5, 5 and 5.5)
> 3', 5', dR and dT Digoxigenin
> 5' Dragonfly Orange®
> 5' DY-(681, 781 and 782)
> dR 6-FAM
> dR and dT HEX
> 5' HiLyte™ Fluor (405, 488, 555, 594, 647, 680 and 750)
> 3', dR and dT JOE
> 5' Marina Blue®
> 5' Oregon Green® (488 and 488 X)
> 5' Pacific Blue™
> 3' QXL®
> 3', 5', dR and dT Rhodamine 6G
> 3', 5', dR and dT ROX
> 5' TAMRA
> dR and dT TET
> 3', 5', dR and dT Texas Red®

The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

Please use your Login or Register Now to directly access your customized EOS interface.



You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
  delivery address product description and reference number
  invoice address quotation number
  telephone number  

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