Synthesis scale vs Guaranteed yield 

The synthesis scale refers to the amount of raw material used to start the synthesis of oligonucleotides. 

The yield corresponds to the amount of final product recovered at the end of the synthesis and purification processes.

Which factors do influence the oligo production yield? 

  • The oligonucleotides length
  • The presence of one or multiple modifications
  • The nature of the modifications
  • The coupling method and efficiency
  • The purification method

Post-synthesis modifications may have a significant impact on the production yield 

Please be aware that post-synthesis modifications may yield 50% less than the stated values.

Post synthesis modifications are listed here :  

  • 5' Alexa Fluor® (350, 430, 488, 500, 514, 532, 546,555, 568,594, 610, 633, 647, 660, 680, 700 and 750)
  • 5' ATTO (390, 425, 465, 488, 495, 520, 532, 550, 565,590, 594, 610, 620, 633, 635, 647N, 655, 680, 700, 725 and 740)
  • 5' BODIPY® (530/550, FL and TR)
  • 3', 5' and dT Cascade Blue®
  • 3' and dT Cy® (3, 3.5, 5 and 5.5)
  • 3', 5', dR and dT Digoxigenin
  • 5' Dragonfly Orange®
  • 5' DY-(681, 781 and 782)
  • dR 6-FAM
  • dR and dT HEX
  • 5' HiLyte™ Fluor (405, 488, 555, 594, 647, 680 and 750)
  • 3', dR and dT JOE
  • 5' Marina Blue®
  • 5' Oregon Green® (488 and 488 X)
  • 5' Pacific Blue™
  • 3' QXL®
  • 3', 5', dR and dT Rhodamine 6G
  • 3', 5', dR and dT ROX
  • 5' TAMRA
  • dR and dT TET
  • 3', 5', dR and dT Texas Red®