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MMPs Assay and ELISA Kits

iD Sensolyte® Assay Kits

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases. They are distinguished from other endopeptidases by their dependence on metal ions as cofactors, their ability to degrade extracellular matrix, and their specific evolutionary DNA sequence. These enzymes are responsible for the breakdown of connective tissues and are important in bone remodeling, the menstrual cycle and repair tissue damages.

MMPs are also thought to play a major role on cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis and host defense1-2.

DescriptionSize Reference USD Qty  
SensoLyte® 390 Generic MMP Activity Kit *Fluorimetric* - 1 kit1 kit AS-72202 374.00Add to cart
SensoLyte® 490 MMP-1 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71128 631.00Add to cart
SensoLyte® 490 MMP-12 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71137 631.00Add to cart
SensoLyte® 490 MMP-13 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71135 631.00Add to cart
SensoLyte® 490 MMP-2 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71129 631.00Add to cart
SensoLyte® 490 MMP-3 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71130 631.00Add to cart
SensoLyte® 490 MMP-7 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71132 631.00Add to cart
SensoLyte® 490 MMP-8 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71133 631.00Add to cart
SensoLyte® 490 MMP-9 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71134 631.00Add to cart
SensoLyte® 520 Generic MMP Activity Kit *Fluorimetric* - 1 kit1 kit AS-71158 457.00Add to cart
SensoLyte® 520 MMP Profiling Kit *Fluorimetric* - 1 kit1 kit AS-71136 691.00Add to cart
SensoLyte® 520 MMP Substrate Sampler Kit *Fluorimetric* - 1 kit1 kit AS-71170 691.00Add to cart
SensoLyte® 520 MMP-1 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71150 457.00Add to cart
SensoLyte® 520 MMP-10 Assay Kit *Fluorimetric* - 1 kit1 kit AS-72024 457.00Add to cart
SensoLyte® 520 MMP-12 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71157 457.00Add to cart
SensoLyte® 520 MMP-13 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71156 457.00Add to cart
SensoLyte® 520 MMP-14 Assay Kit *Fluorimetric* - 1 kit1 kit AS-72025 457.00Add to cart
SensoLyte® 520 MMP-2 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71151 457.00Add to cart
SensoLyte® 520 MMP-3 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71152 457.00Add to cart
SensoLyte® 520 MMP-7 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71153 457.00Add to cart
SensoLyte® 520 MMP-8 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71154 457.00Add to cart
SensoLyte® 520 MMP-9 Assay Kit *Fluorimetric* - 1 kit1 kit AS-71155 457.00Add to cart
SensoLyte® 570 Generic MMP Assay Kit *Fluorimetric* - 1 kit1 kit AS-72101 457.00Add to cart
SensoLyte® Generic MMP Assay Kit *Colorimetric* - 1 kit1 kit AS-72095 362.00Add to cart
SensoLyte® Plus 520 MMP-1 Assay Kit *Fluorimetric and Enhanced Selectivity* - 1 kit1 kit AS-72012 589.00Add to cart
SensoLyte® Plus 520 MMP-13 Assay Kit *Fluorimetric and Enhanced Selectivity* - 1 kit1 kit AS-72019 589.00Add to cart
SensoLyte® Plus 520 MMP-2 Assay Kit *Fluorimetric and Enhanced Selectivity* - 1 kit1 kit AS-72224 589.00Add to cart
SensoLyte® Plus 520 MMP-9 Assay Kit *Fluorimetric and Enhanced Selectivity* - 1 kit1 kit AS-72017 589.00Add to cart


Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases. They are distinguished from other endopeptidases by their dependence on metal ions as cofactors, their ability to degrade extracellular matrix, and their specific evolutionary DNA sequence. These enzymes are responsible for the breakdown of connective tissues and are important in bone remodeling, the menstrual cycle and repair tissue damages.

MMPs are also thought to play a major role on cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis and host defense1-2.


Benefits



• Convenient format: - Most kits are provided in bulk (sufficient to perform 100 assays in a 96-well plate format)
• Enhanced value: - With superior fluorescence quantum yield and longer emission wavelength, both FRET peptides are less interfered by the autofluorescence
• High Speed: - The entire assay process can be completed in a minimal time
• Assured Reliability: - A detailed protocol and references are provided

Description



λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Generic MMP Assay Kit
SensoLyte® 570 Generic MMP
Fluorimetric
540/575 50 μl cell sup. 1 ng 5-TAMRA/QXL™ 570 FRET peptide • 5-TAMRA, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop Solution 1 hour 100
SensoLyte 520 Generic MMP
Fluorimetric
490/520 50 μl cell sup. 1 ng

5-FAM/QXL™ 520 FRET peptide • 5-FAM, fluorescence reference standard
• APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop Solution

1 hour 100
SensoLyte® Generic MMP
Colorimetric
Abs
412
(with thiol reactive Ellmon’s reagent)
HTP n.d. MMP colorimetric substrate • Reference standard • Assay Buffer • MMP inhibitor • Trypsin • Trypsin inhibitor • Stop Solution Minimal 100
SensoLyte® 520 MMP Profiling Kit Fluorimetric 490/520 50 μl n.a. 2 Microplates coated with 16 5-FAM/QXL™ 520 FRET peptide substrates of MMPs • 5-FAM-Pro-Leu-OH, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 2 x 96-well plates
SensoLyte® 520 MMP Substrate Sampler Kit Fluorimetric 490/520 50 μl n.a. 16 different MMP 5-FAM/QXL™ 520 FRET peptide substrates • 5-FAM-Pro-
Leu-OH, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution
Minimal 16 x 20 assays
SensoLyte® 390 Generic MMP Fluorimetric 330/390 5-50 µl nd Mca/Dnp, MMP substrate • Mca Fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution 60 min 100
MMP-1 (interstitial collagenase, fibroblast collagenase)
SensoLyte® PLUS 520 MMP-1
Fluorimetric
490/520 100 μl 30 pg Microplate coated with monoclonal anti human MMP-1 antibody (12 x 8 black strips) • MMP-1 standard, recombinant human pro-MMP-1 • MMP dilution buffer • 10X Wash buffer • APMA, 4-aminophenylmercuric acetate • MMP-1 substrate: 5 FAM/QXL™520 FRET peptide • Assay buffer • Stop solution • Adhesive cover strip (3 sheets) Minimal 96-well plate
SensoLyte® 520 MMP-1
Fluorimetric
490/520 50 μl cell sup. 0.5 ng MMP-1 substrate: 5-FAM/QXL™520 FRET peptide (susceptible to MMP-2, 3, 8, 12, and 13) • 5-FAM-Pro-Leu-OH, fluorescence reference standard
• APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution
Minimal 100
SensoLyte® 490 MMP-1
Fluorimetric
340/490 50 μl cell sup. 3.6 ng MMP-1 substrate : EDANS/DabcylPlus™ FRET peptide • EDANS, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 500
MMP-2 (gelatinase A, degrades type IV collagen)
SensoLyte® 520 MMP-2 Assay Kit Fluorimetric 490/520 50 μl cell sup. 1.5 ng MMP-2 substrate: 5-FAM/QXL™520 FRET peptide (susceptible to MMP-8, 12, 13 and 14) • 5-FAM-Pro-Leu-OH, fluorescence reference standard
• APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution
Minimal 100
SensoLyte® 490 MMP-2 Assay Kit Fluorimetric 340/490 50 μl cell sup. n.d. MMP-2 substrate: EDANS/DABSYL FRET peptide (susceptible to MMP-8, 12, 13 and 14) • EDANS, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 500
SensoLyte® PLUS 520 MMP-2 Fluorimetric 490/520 100 μl 1 ng Microplate coated with monoclonal anti human MMP-2 antibody (12 x 8 black strips) • MMP-2 standard, recombinant human pro-MMP-2 • Assay buffer • 10X Wash buffer • APMA, 4-aminophenylmercuric acetate • MMP-2 substrate: 5 FAM/QXL™520 FRET peptide • Stop solution • Adhesive cover strip Minimal 100
MMP-3 (stromelysin-1)
SensoLyte® 520 MMP-3 Assay Kit
Fluorimetric
490/520 50 μl cell sup. 2 ng MMP-3 substrate: 5-FAM/QXL™520 FRET peptide (susceptible to MMP-1, 2, 8, 12 and 13 ) • 5-FAM-Pro-Leu-OH, fluorescence reference standard
• APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution
Minimal 100
SensoLyte® 490 MMP-3 Assay Kit
Fluorimetric
340/490 50 μl cell sup. n.d. MMP-3 substrate: EDANS/DABSYL FRET peptide (susceptible to MMP-1, 2, 8, 12 and 13) • EDANS, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 500
MMP-7 (matrisilysin,PUMP) Assay Kits
SensoLyte® 520 MMP-7
Fluorimetric
490/520 50 μl cell sup. 1 ng MMP-7 substrate: 5-FAM/QXL™520 FRET peptide (can also be cleaved by MMP-12 and 13, but not by MMP-1, 2, 3, 8, 9, and 14) • 5-FAM-Pro-Leu-OH, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 100
SensoLyte® 490 MMP-7
Fluorimetric
340/490 50 μl cell sup. n.d. MMP-7 substrate: EDANS/DABSYL FRET peptide can also be cleaved by MMP-12 and 13, but not by MMP-1, 2, 3, 8, 9, and 14) • EDANS, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 500
MMP-8 (collagenase-2, neutrophil collagenase) Assay Kits
SensoLyte® 520 MMP-8
Fluorimetric
490/520 50 μl cell sup. 0.2 ng MMP-8 substrate: 5-FAM/QXL™520 FRET peptide (susceptible to MMP- 2, 12, 13 and 14) • 5-FAM-Pro-Leu-OH, fluorescence reference standard
• APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution
Minimal 100
SensoLyte® 490 MMP-8
Fluorimetric
340/490 50 μl cell sup. 3.5 ng MMP-8 substrate: EDANS/DABSYL FRET peptide (susceptible to MMP- 2, 12, 13 and 14) • EDANS, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 500
MMP-9 (gelatinase, collagenase) Assay Kits
SensoLyte® PLUS 520 MMP-9
Fluorimetric
490/520 100 μl 0.3 ng Microplate coated with monoclonal anti human MMP-9 antibody (12 x 8 black strips) • MMP-9 standard, recombinant human pro-MMP-9 • MMP dilution buffer • 10X Wash buffer • APMA, 4-aminophenylmercuric acetate • MMP-9 substrate:5-FAM/QXL™520 FRET peptide • Assay buffer • Stop solution
• Adhesive cover strip (3 sheets)
Minimal 96-well plate
SensoLyte® 520 MMP-9
Fluorimetric
490/520 50 μl cell sup. 20 pg MMP-9 substrate: 5-FAM/QXL™520 FRET peptide (can also be cleaved by MMP-1, 2, 3, 7, 8, 12, 13, and 14) • 5-FAM-Pro-Leu-OH, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 100
SensoLyte® 490 MMP-9
Fluorimetric
340/490 50 μl cell sup. n.d. MMP-9 substrate: EDANS/DABSYL FRET peptide (can also be cleaved by MMP-1, 2, 3, 7, 8, 12, 13, and 14) • EDANS, fluorescence reference standard
• APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution
Minimal 500
MMP-10 (stromelysin) Assay Kits
SensoLyte® 520 MMP-10
Fluorimetric
490/520 50 μl cell sup. 0.8 ng MMP-10 substrate: 5-FAM/QXL™520 FRET peptide (can also be cleaved by MMP-7, 12 and 13) • 5-FAM-Pro-Leu-OH, fluorescence reference standard
• APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution
Minimal 100
MMP-12 (macrophage elastase) Assay Kits
SensoLyte® 520 MMP-12
Fluorimetric
490/520 50 μl cell sup. 1 ng MMP-12 substrate: 5-FAM/QXL™520 FRET peptide (can also be cleaved by MMP- 3, but not MMP-1, 2, 7, 8, 9, 13, and 14) • 5-FAM-Pro-Leu-OH, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate
• Assay buffer • Stop solution
Minimal 100
SensoLyte® 490 MMP-12
Fluorimetric
340/490 50 μl cell sup. 4.3 ng MMP-12 substrate: EDANS/DABSYL FRET peptide (can also be cleaved by MMP- 3, but not MMP-1, 2, 7, 8, 9, 13, and 14) • EDANS, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 500
MMP-13 (collagenase-3) Assay Kits
SensoLyte® PLUS 520 MMP-13
Fluorimetric
490/520 100 μl 0.3 ng Microplate coated with monoclonal anti human MMP-13 antibody (12 x 8 black strips) • MMP-13 standard, recombinant human pro-MMP-13 • MMP dilution buffer • 10X Wash buffer • APMA, 4-aminophenylmercuric acetate • MMP-13 substrate:5-FAM/QXL™520 FRET peptide • Assay buffer • Stop solution • Adhesive cover strip (3 sheets) Minimal 96-well plate
SensoLyte® 520 MMP-13
Fluorimetric
490/520 50 μl cell sup. 3 ng MMP-13 substrate: 5-FAM/QXL™520 FRET peptide (the cleavage rate of MMP-13 on this FRET substrate is 5-50 fold faster rate than those of MMP-1, 2, 3, 7, 8, 9, 12, and 14) • 5-FAM-Pro-Leu-OH, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 100
SensoLyte® 490 MMP-13
Fluorimetric
340/490 50 μl cell sup. n.d. MMP-13 substrate: EDANS/DABSYL FRET peptide the cleavage rate of MMP-13 on this FRET substrate is 5-50 fold faster rate than those of MMP-1, 2, 3, 7, 8, 9, 12, and 14) • EDANS, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 500
MMP-14 (membrane-type MMP, MT1-MMP)
SensoLyte® 520 MMP-14
Fluorimetric
490/520 50 μl cell sup. 0.2 ng MMP-14 substrate: 5-FAM/QXL™520 FRET peptide (can also be cleaved by MMP- 1, 2, 7, 8, 12, and 13) • 5-FAM-Pro-Leu-OH, fluorescence reference standard • APMA, 4-aminophenylmercuric acetate • Assay buffer • Stop solution Minimal 100
MMP-15
SensoLyte® 520 MMP-15 Fluorimetric 490/520 50 μl sample 0.78 ng MMP-15 substrate: 5-FAM/QXL™ 520 FRET peptide • 5-FAM-Pro-Leu-OH, fluorescence reference standard • Mersalylic acid • Assay buffer • Stop solution Minimal 100
MMP-16
SensoLyte® 520 MMP-16 Fluorimetric 490/520 50 μl sample nd MMP-16 substrate: 5-FAM/QXL™ 520 FRET peptide • 5-FAM-Pro-Leu-OH, fluorescence reference standard • MMP-16 Catalytic Domain, Human recombinant, E. coli · MMP-16 Inhibitor • Assay buffer • Stop solution Minimal 100
MMP-24
SensoLyte® 520 MMP-24 Fluorimetric 490/520 50 μl sample 1 ng MMP-24 substrate: 5-FAM/QXL™ 520 FRET peptide • 5-FAM-Pro-Leu-OH, fluorescence reference standard • MMP-24 Catalytic Domain, Human recombinant, E. coli · MMP-24 Inhibitor • Assay buffer • Stop solution Minimal 100

sup. = supernatant

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