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One step qRT-PCR MasterMix

qRT-PCR kits for Probe assay for ABI Step One

Takyon Kits

The One step RT qPCR MasterMix contains enough reagents for up to 600 - 25 µl reactions using the hotstart enzyme, HotGoldStar and has been validated on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.


DescriptionSize Reference USD Qty  
One step qRT-PCR 2X MasterMix 7.5 ml - 5 x 600 rxn (5 x 7.5 ml)5 x 600 rxn (5 x 7.5 ml) 05-QPRT-032X 3 409.00Add to cart
One step qRT-PCR 2X MasterMix 7.5 ml - 10 x 600 rxn (10 x 7.5 ml)10 x 600 rxn (10 x 7.5 ml) 10-QPRT-032X 5 541.00Add to cart
One step qRT-PCR 2X MasterMix 7.5 ml - 600 rxn (7.5 ml)600 rxn (7.5 ml) RT-QPRT-032X 852.00Add to cart
25 µl reactions

Delivery times

   2 WD if ordered before Thursday

Shipping conditions

   Dry ice

Storage conditions


For long term storage the One step RT qPCR MasterMix Plus should be stored at -65 °C to -75 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 1 year.
For mid term storage the One step RT qPCR MasterMix Plus can be stored at -15 °C to -25 °C for 6 months.For short term storage all components of the One step RT qPCR MasterMix, except the tube of Euroscript / RNase Inhibitor, can be stored at 4 °C for one month.
The Euroscript / RNase Inhibitor has to be stored at -15 °C to -25 °C.

One step RT qPCR MasterMix picture

Our One step RT qPCR MasterMix, will allow you to achieve reliable, consistent and accurate Real-Time RT qPCR results for probe detection. The One step RT qPCR MasterMix contains enough reagents for up to 600 - 25 µl reactions using the hotstart enzyme, HotGoldStar and has been validated on ABI prism® 5700, 7000, 7700, 7900, 7300, MasterCycler® ep realplex. The 2x buffer contains dNTP, HotGoldStar, MgCl2, stabilizers and ROX passive reference. Our EuroSript/RNase Inhibitor is provided in a separate tube. An extra tube of 50 mM MgCl2 is provided in the kit to optimize MgCl2 concentration if necessary.


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Certificate of analysis


To obtain the Certificate of Analysis corresponding to your qPCR kit of interest please contact us via email and display the reference code of the qPCR kit and the batch number. Contact us

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