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Peptidyl-prolyl isomerase Pin1 Assay Kit

iD Sensolyte® Assay Kits

Peptidyl-prolyl isomerase (PPIase) Pin1 catalyzes cis/trans isomerization of the phospho-Serine/Threonine-Proline peptide bonds. Pin1 has many biological substrates, plays critical role in cell-cycle regulation, and up-regulated in many human cancers. 1-5 The SensoLyte® Green Pin1 Assay Kit uses fluorogenic substrate that was pretreated to convert into cis isoform. Increase in fluorescence intensity is directly proportional to the Pin1 activity.

Optimized Performance: This kit is optimized to detect Pin-1 activity
Enhanced Value: It provides enough reagents to perform 100 assays in a 96-well format
High Speed: The entire process can be completed in 1 hour
Assured Reliability: Detailed protocol and references are provided


DescriptionSize Reference USD Qty  
SensoLyte® Green PIN1 Activity Assay Kit *Fluorimetric* - 1 kit 1 kit AS-72240 501.00Add to cart


Other Materials Required (but not provided)

  • 96-well microplate: Black, flat bottom 96-well plate with non-binding surface
  • Fluorescence microplate reader: Capable of detecting emission at 520 nm with excitation at 490 nm
  • Plate cover: To prevent liquid evaporation during incubation step

Storage and Handling

  • Store kit Components A, C, D, and E at -20°C. Store Component B at -80°C
  • Protect Component A from light and from moisture

Peptidyl-prolyl isomerase (PPIase) Pin1 catalyzes cis/trans isomerization of the phospho-Serine/Threonine-Proline peptide bonds.1-5 Pin1 consists of short N-terminal protein-protein interaction domain that allows enzyme to bind phosphoproteins and longer C-terminal izomerase domain.1-5 Pin1 has many biological substrates, plays critical role in cell-cycle regulation, and up-regulated in many human cancers. 1-5 Recently, Pin1 was linked to the Alzheimer’s disease pathogenesis.1,2,4 Pin1 can bind phosphorylated Thr-212/231 residues of Tau protein and increase its deposphorylation that may prevent formation of Tau neurofibrilary tangles. 1,2,4

The SensoLyte® Green Pin1 Assay Kit uses fluorogenic substrate that was pretreated to convert into cis isoform. Pin1 changes substrate into trans conformation that is readily cleaved to generate fluorescent signal which can be monitored at Ex/Em=490/520nm. Increase in fluorescence intensity is directly proportional to the Pin1 activity.

References

  • 1. Kimura T. et.al., J Biol Chem 288, 11 (2013): 7968-7977
  • 2. Galas M-C., et.al., J Biol Chem 281, 28 (2006): 19296-10394
  • 3. Moore J.D., et.al., Biorg and Med Chem Let 23 (2013): 4283-4291
  • 4. Dong L., et.al., Biorg and Med Chem Let 20 (2010): 2210-2214
  • 5. Urusova D.V., et.al., Cancer Prev Res 4, 9 (2011): 1366-1377
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