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Peroxidase/Hydrogen Peroxide Assay kits

iD Sensolyte® Assay Kits

Peroxidases catalyze oxidation-reduction reactions and play an important role in protecting cell from oxidative injury. HRP-protein conjugates are extensively used as secondary detection reagents in ELISA, immunohistochemistry and assay development. Hydrogen peroxide is involved in a number of biological events; in particular; free radical-induced biochemical reactions. 

The SensoLyte® ADHP Peroxidase Assay Kit uses highly purified ADHP to quantify peroxidase activity in solutions, in cell extracts and in live cells and on solid surfaces (such as PVDF membranes). The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors. 

The SensoLyte® Luminescent Peroxidase Assay Kit provides highly sensitive chemiluminescent substrate to quantify peroxidase activity in solutions, in cell extracts, in live cells. 

The SensoLyte® Luminescent Peroxidase ELISA Assay Kit provides a highly sensitive chemiluminescent substrate for quantifying HRP activity in ELISA. 

The SensoLyte® ADHP Peroxidase ELISA Assay Kits use highly purified ADHP to quantify HRP activity in the secondary antibody or streptavidin-HRP conjugates. They have been used to detect as low as femtomolar (10-15 M) concentration of test antibodies in an ELISA format. 

The SensoLyte® ADHP Hydrogen Peroxide Assay Kit uses highly purified ADHP to quantify hydrogen peroxide in solutions; in cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions.

DescriptionSize Reference USD Qty  
SensoLyte® ADHP Hydrogen Peroxide Assay Kit *Fluorimetric* - 1 kit1 kit AS-71112 200.00Add to cart
SensoLyte® ADHP Peroxidase Assay Kit *Fluorimetric* - 1 kit1 kit AS-71111 200.00Add to cart
SensoLyte® Luminescent Peroxidase Assay Kit *Luminometric* - 1 kit1 kit AS-72127 339.00Add to cart
SensoLyte® Luminescent Peroxidase ELISA Assay Kit *Luminometric* - 1 kit1 kit AS-72128 339.00Add to cart


Description

λ
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Peroxidase
SensoLyte® ADHP Peroxidase ELISA Fluorimetric Ex/Em 573/590nm upon oxidation
Used for ELISA Used for ELISA • ADHP (10-Acetyl-3, 7-dihydroxyphenoxazine), fluorogenic peroxidase substrate
• H2O2 complex
• Assay buffer
• HRP-conjugated goat anti-rabbit OR mouse IgG
15-30 min 500
SensoLyte® Luminescent Peroxidase ELISA Fluorimetric Luminescence Used for ELISA Used for ELISA • Substrate A
• Substrate B
• Assay buffer
30 min 500
SensoLyte® ADHP Peroxidase Assay Kit Fluorimetric Ex/Em 573/590nm upon oxidation 50 µl n.d • ADHP (10-Acetyl-3, 7-dihydroxyphenoxazine) Peroxidase substrate
• H2O2 standard
• Assay buffer
30 min 500
SensoLyte®  Luminescent Peroxidase Luminometric  Luminescence 50 µl 1 fg • Substrate A
• Substrate B
• Peroxidase ( Horseradish Peroxidase) standard
• Assay buffer
30-60 min 500
Hydrogen Peroxide
SensoLyte® ADHP Hydrogen Peroxide Assay Kit Fluorimetric Ex/Em 573/590nm upon oxidation 50 µl 1 μM
(0.1 nmol) with a linear range up to 20 μM
(2 nmol)
•ADHP (10-Acetyl-3, 7-dihydroxyphenoxazine)
• H2O2 standard
• Assay buffer
• HRP, Horseradish peroxidase
30-60 min 500
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